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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K6 ASK2, MAPKKK6, MEKK6
Sequence Length (AA) Molecular Weight (Da)
1288 142596
Protein Name
Mitogen-activated protein kinase kinase kinase 6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGPCPRSGA ERAGSCWQDP LAVALSRGRQ LAAPPGRGCA RSRPLSVVYV
60 70 80 90 100
LTREPQPGLE PREGTEAEPL PLRCLREACA QVPRPRPPPQ LRSLPFGTLE
110 120 130 140 150
LGDTAALDAF YNADVVVLEV SSSLVQPSLF YHLGVRESFS MTNNVLLCSQ
160 170 180 190 200
ADLPDLQALR EDVFQKNSDC VGSYTLIPYV VTATGRVLCG DAGLLRGLAD
210 220 230 240 250
GLVQAGVGTE ALLTPLVGRL ARLLEATPTD SCGYFRETIR RDIRQARERF
260 270 280 290 300
SGPQLRQELA RLQRRLDSVE LLSPDIIMNL LLSYRDVQDY SAIIELVETL
310 320 330 340 350
QALPTCDVAE QHNVCFHYTF ALNRRNRPGD RAKALSVLLP LVQLEGSVAP
360 370 380 390 400
DLYCMCGRIY KDMFFSSGFQ DAGHREQAYH WYRKAFDVEP SLHSGINAAV
410 420 430 440 450
LLIAAGQHFE DSKELRLIGM KLGCLLARKG CVEKMQYYWD VGFYLGAQIL
460 470 480 490 500
ANDPTQVVLA AEQLYKLNAP IWYLVSVMET FLLYQHFRPT PEPPGGPPRR
510 520 530 540 550
AHFWLHFLLQ SCQPFKTACA QGDQCLVLVL EMNKVLLPAK LEVRGTDPVS
560 570 580 590 600
TVTLSLLEPE TQDIPSSWTF PVASICGVSA SKRDERCCFL YALPPAQDVQ
610 620 630 640 650
LCFPSVGHCQ WFCGLIQAWV TNPDSTAPAE EAEGAGEMLE FDYEYTETGE
660 670 680 690 700
RLVLGKGTYG VVYAGRDRHT RVRIAIKEIP ERDSRFSQPL HEEIALHRRL
710 720 730 740 750
RHKNIVRYLG SASQGGYLKI FMEEVPGGSL SSLLRSVWGP LKDNESTISF
760 770 780 790 800
YTRQILQGLG YLHDNHIVHR DIKGDNVLIN TFSGLLKISD FGTSKRLAGI
810 820 830 840 850
TPCTETFTGT LQYMAPEIID QGPRGYGKAA DIWSLGCTVI EMATGRPPFH
860 870 880 890 900
ELGSPQAAMF QVGMYKVHPP MPSSLSAEAQ AFLLRTFEPD PRLRASAQTL
910 920 930 940 950
LGDPFLQPGK RSRSPSSPRH APRPSDAPSA SPTPSANSTT QSQTFPCPQA
960 970 980 990 1000
PSQHPPSPPK RCLSYGGTSQ LRVPEEPAAE EPASPEESSG LSLLHQESKR
1010 1020 1030 1040 1050
RAMLAAVLEQ ELPALAENLH QEQKQEQGAR LGRNHVEELL RCLGAHIHTP
1060 1070 1080 1090 1100
NRRQLAQELR ALQGRLRAQG LGPALLHRPL FAFPDAVKQI LRKRQIRPHW
1110 1120 1130 1140 1150
MFVLDSLLSR AVRAALGVLG PEVEKEAVSP RSEELSNEGD SQQSPGQQSP
1160 1170 1180 1190 1200
LPVEPEQGPA PLMVQLSLLR AETDRLREIL AGKEREYQAL VQRALQRLNE
1210 1220 1230 1240 1250
EARTYVLAPE PPTALSTDQG LVQWLQELNV DSGTIQMLLN HSFTLHTLLT
1260 1270 1280 1288
YATRDDLIYT RIRGGMVCRI WRAILAQRAG STPVTSGP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-851 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K6
Peptide Modified Sequence
C[+57.0]LSYGGTSQLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
962
Peptide - Site of Modification
1
Peptide Start
962
Peptide End
972
CPTAC ID
CPTAC-851
Peptide Molecular Mass
1,240.5870
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13.2 9.3 9.9 11.1 9.6 9.4 17.2 13.4 13.7 15 15 15
y7 (1+) 5.5 3.2 4.4 5.8 3.6 4.7 8 4.8 6.4 15 15 15
y8 (1+) 8.6 6.6 5.6 8.8 6.2 5 12.3 9.1 7.5 15 15 15
y9 (1+) 5.4 3.6 3 5.3 4.2 2.6 7.6 5.5 4 15 15 15
b3 (1+) 16.9 7.9 8.3 16.6 7.8 6.3 23.7 11.1 10.4 15 15 15
sum 3 2 2.4 3.3 2.6 2.3 4.5 3.3 3.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-852 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K6
Peptide Sequence
FSGPQLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
250
Peptide End
256
CPTAC ID
CPTAC-852
Peptide Molecular Mass
803.4290
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7.1 7.1 7.7 6.9 8.2 6.8 9.9 10.8 10.3 15 15 15
y4 (1+) 8.9 7.3 5.3 9.6 6.9 6.2 13.1 10 8.2 15 15 15
y5 (1+) 3.4 3.8 3.9 2.9 3.7 3.5 4.5 5.3 5.2 15 15 15
y6 (1+) 5.5 3.6 2 8.5 4.5 2.6 10.1 5.8 3.3 15 15 15
sum 3 1.9 1.8 4 2.5 2 5 3.1 2.7 15 15 15

Additional Resources and Comments