MAP3K6, CPTAC-851 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP3K6	ASK2, MAPKKK6, MEKK6

Sequence Length (AA)	Molecular Weight (Da)
1288	142596

Protein Name
Mitogen-activated protein kinase kinase kinase 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGPCPRSGA	ERAGSCWQDP	LAVALSRGRQ	LAAPPGRGCA	RSRPLSVVYV
60	70	80	90	100
LTREPQPGLE	PREGTEAEPL	PLRCLREACA	QVPRPRPPPQ	LRSLPFGTLE
110	120	130	140	150
LGDTAALDAF	YNADVVVLEV	SSSLVQPSLF	YHLGVRESFS	MTNNVLLCSQ
160	170	180	190	200
ADLPDLQALR	EDVFQKNSDC	VGSYTLIPYV	VTATGRVLCG	DAGLLRGLAD
210	220	230	240	250
GLVQAGVGTE	ALLTPLVGRL	ARLLEATPTD	SCGYFRETIR	RDIRQARERF
260	270	280	290	300
SGPQLRQELA	RLQRRLDSVE	LLSPDIIMNL	LLSYRDVQDY	SAIIELVETL
310	320	330	340	350
QALPTCDVAE	QHNVCFHYTF	ALNRRNRPGD	RAKALSVLLP	LVQLEGSVAP
360	370	380	390	400
DLYCMCGRIY	KDMFFSSGFQ	DAGHREQAYH	WYRKAFDVEP	SLHSGINAAV
410	420	430	440	450
LLIAAGQHFE	DSKELRLIGM	KLGCLLARKG	CVEKMQYYWD	VGFYLGAQIL
460	470	480	490	500
ANDPTQVVLA	AEQLYKLNAP	IWYLVSVMET	FLLYQHFRPT	PEPPGGPPRR
510	520	530	540	550
AHFWLHFLLQ	SCQPFKTACA	QGDQCLVLVL	EMNKVLLPAK	LEVRGTDPVS
560	570	580	590	600
TVTLSLLEPE	TQDIPSSWTF	PVASICGVSA	SKRDERCCFL	YALPPAQDVQ
610	620	630	640	650
LCFPSVGHCQ	WFCGLIQAWV	TNPDSTAPAE	EAEGAGEMLE	FDYEYTETGE
660	670	680	690	700
RLVLGKGTYG	VVYAGRDRHT	RVRIAIKEIP	ERDSRFSQPL	HEEIALHRRL
710	720	730	740	750
RHKNIVRYLG	SASQGGYLKI	FMEEVPGGSL	SSLLRSVWGP	LKDNESTISF
760	770	780	790	800
YTRQILQGLG	YLHDNHIVHR	DIKGDNVLIN	TFSGLLKISD	FGTSKRLAGI
810	820	830	840	850
TPCTETFTGT	LQYMAPEIID	QGPRGYGKAA	DIWSLGCTVI	EMATGRPPFH
860	870	880	890	900
ELGSPQAAMF	QVGMYKVHPP	MPSSLSAEAQ	AFLLRTFEPD	PRLRASAQTL
910	920	930	940	950
LGDPFLQPGK	RSRSPSSPRH	APRPSDAPSA	SPTPSANSTT	QSQTFPCPQA
960	970	980	990	1000
PSQHPPSPPK	RCLSYGGTSQ	LRVPEEPAAE	EPASPEESSG	LSLLHQESKR
1010	1020	1030	1040	1050
RAMLAAVLEQ	ELPALAENLH	QEQKQEQGAR	LGRNHVEELL	RCLGAHIHTP
1060	1070	1080	1090	1100
NRRQLAQELR	ALQGRLRAQG	LGPALLHRPL	FAFPDAVKQI	LRKRQIRPHW
1110	1120	1130	1140	1150
MFVLDSLLSR	AVRAALGVLG	PEVEKEAVSP	RSEELSNEGD	SQQSPGQQSP
1160	1170	1180	1190	1200
LPVEPEQGPA	PLMVQLSLLR	AETDRLREIL	AGKEREYQAL	VQRALQRLNE
1210	1220	1230	1240	1250
EARTYVLAPE	PPTALSTDQG	LVQWLQELNV	DSGTIQMLLN	HSFTLHTLLT
1260	1270	1280	1288
YATRDDLIYT	RIRGGMVCRI	WRAILAQRAG	STPVTSGP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-851 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K6
Peptide Modified Sequence: C[+57.0]LSYGGTSQLR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 962
Peptide - Site of Modification: 1
Peptide Start: 962
Peptide End: 972

CPTAC ID: CPTAC-851
Peptide Molecular Mass: 1,240.5870
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	13.2	9.3	9.9	11.1	9.6	9.4	17.2	13.4	13.7	15	15	15
y7 (1+)	5.5	3.2	4.4	5.8	3.6	4.7	8	4.8	6.4	15	15	15
y8 (1+)	8.6	6.6	5.6	8.8	6.2	5	12.3	9.1	7.5	15	15	15
y9 (1+)	5.4	3.6	3	5.3	4.2	2.6	7.6	5.5	4	15	15	15
b3 (1+)	16.9	7.9	8.3	16.6	7.8	6.3	23.7	11.1	10.4	15	15	15
sum	3	2	2.4	3.3	2.6	2.3	4.5	3.3	3.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-852 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K6
Peptide Sequence: FSGPQLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 250
Peptide End: 256

CPTAC ID: CPTAC-852
Peptide Molecular Mass: 803.4290
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend