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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K7 TAK1
Sequence Length (AA) Molecular Weight (Da)
606 67196
Protein Name
Mitogen-activated protein kinase kinase kinase 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTASAASSS SSSSAGEMIE APSQVLNFEE IDYKEIEVEE VVGRGAFGVV
60 70 80 90 100
CKAKWRAKDV AIKQIESESE RKAFIVELRQ LSRVNHPNIV KLYGACLNPV
110 120 130 140 150
CLVMEYAEGG SLYNVLHGAE PLPYYTAAHA MSWCLQCSQG VAYLHSMQPK
160 170 180 190 200
ALIHRDLKPP NLLLVAGGTV LKICDFGTAC DIQTHMTNNK GSAAWMAPEV
210 220 230 240 250
FEGSNYSEKC DVFSWGIILW EVITRRKPFD EIGGPAFRIM WAVHNGTRPP
260 270 280 290 300
LIKNLPKPIE SLMTRCWSKD PSQRPSMEEI VKIMTHLMRY FPGADEPLQY
310 320 330 340 350
PCQYSDEGQS NSATSTGSFM DIASTNTSNK SDTNMEQVPA TNDTIKRLES
360 370 380 390 400
KLLKNQAKQQ SESGRLSLGA SRGSSVESLP PTSEGKRMSA DMSEIEARIA
410 420 430 440 450
ATTAYSKPKR GHRKTASFGN ILDVPEIVIS GNGQPRRRSI QDLTVTGTEP
460 470 480 490 500
GQVSSRSSSP SVRMITTSGP TSEKPTRSHP WTPDDSTDTN GSDNSIPMAY
510 520 530 540 550
LTLDHQLQPL APCPNSKESM AVFEQHCKMA QEYMKVQTEI ALLLQRKQEL
560 570 580 590 600
VAELDQDEKD QQNTSRLVQE HKKLLDENKS LSTYYQQCKK QLEVIRSQQQ
606
KRQGTS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-853 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K7
Peptide Sequence
DLKPPNLLLVAGGTVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
156
Peptide End
172
CPTAC ID
CPTAC-853
Peptide Molecular Mass
1,747.0608
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 7.5 13.9 8.4 12.4 14.2 9.3 14.5 19.9 12.5 15 15 15
y7 (1+) 17.9 11.4 7.9 18 10 8.3 25.4 15.2 11.5 15 15 15
y8 (1+) 17.9 12.4 7.4 15.5 14.5 8.6 23.7 19.1 11.3 15 15 15
y9 (1+) 29.4 14.1 7.4 24.9 15.8 11 38.5 21.2 13.3 15 15 15
b8 (1+) 28.3 13.8 10.5 24.5 12 10.6 37.4 18.3 14.9 15 15 15
b7 (1+) 12.5 8.8 8.2 11.4 11.5 8.1 16.9 14.5 11.5 15 15 15
b6 (1+) 24.9 7.6 7.4 24.7 11.5 7.8 35.1 13.8 10.8 15 15 15
b4 (1+) 42.1 23.9 14.1 37 24.6 14.9 56 34.3 20.5 15 15 15
sum 9.1 5.8 2.9 8.5 5.7 2.6 12.5 8.1 3.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-854 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K7
Peptide Sequence
IAATTGNGQPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
11
CPTAC ID
CPTAC-854
Peptide Molecular Mass
1,084.5625
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 13 10.3 4.1 16.2 10.1 4.3 20.8 14.4 5.9 15 15 15
y5 (1+) 10.8 8.9 5 9.4 9.7 5.1 14.3 13.2 7.1 15 15 15
y6 (1+) 5 3.7 2.1 6.3 3.7 3.6 8 5.2 4.2 15 15 15
y7 (1+) 6 4.5 4.6 6.5 3.7 4.9 8.8 5.8 6.7 15 15 15
y8 (1+) 4.4 4.4 5.5 5.9 5.3 3.1 7.4 6.9 6.3 15 15 15
y9 (1+) 5.6 4.9 2.6 5.3 6.2 2.7 7.7 7.9 3.7 15 15 15
y10 (1+) 6.7 4.2 4 7.8 4.5 5.4 10.3 6.2 6.7 15 15 15
sum 2.4 2.5 1.1 2.7 3 1.3 3.6 3.9 1.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-855 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K7
Peptide Modified Sequence
MS[+80.0]ADMSEIEAR
Modification Type
Phospho (ST)
Protein - Site of Modification
389
Peptide - Site of Modification
2
Peptide Start
388
Peptide End
398
CPTAC ID
CPTAC-855
Peptide Molecular Mass
1,318.4935
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 38.3 16.2 26.6 40.8 17 27.5 56 23.5 38.3 15 15 15
y5 (1+) 34.8 16 14.4 28.5 19 13.9 45 24.8 20 15 15 15
y6 (1+) 16 6.7 5.9 13.3 8.9 6.8 20.8 11.1 9 15 15 15
y7 (1+) 15.8 12.4 5.8 14.2 12.4 7.9 21.2 17.5 9.8 15 15 15
sum 9.3 6.6 5.1 8.1 7.8 4.8 12.3 10.2 7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-856 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K7
Peptide Sequence
MSADMSEIEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
388
Peptide End
398
CPTAC ID
CPTAC-856
Peptide Molecular Mass
1,238.5271
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.1 7 5.7 9.3 4.8 5.9 14.5 8.5 8.2 15 15 15
y5 (1+) 10 10.8 4.3 13 11.7 4.8 16.4 15.9 6.4 15 15 15
y6 (1+) 6.6 4.2 4.8 4.7 4.5 4.9 8.1 6.2 6.9 15 15 15
y7 (1+) 8.6 4.3 2.4 7.2 5.8 3.2 11.2 7.2 4 15 15 15
y8 (1+) 8.5 4.3 4 8.8 6.1 4.3 12.2 7.5 5.9 15 15 15
y9 (1+) 10.8 5.1 6.6 10.4 5 7.6 15 7.1 10.1 15 15 15
y10 (1+) 10.9 4.6 5.9 9.5 6.3 8 14.5 7.8 9.9 15 15 15
b4 (1+) 18.3 12.1 8.3 17.2 13.3 8.3 25.1 18 11.7 15 15 15
sum 3 1.6 1.8 3.2 1.7 2 4.4 2.3 2.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1048 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K7
Peptide Modified Sequence
S[+80.0]IQDLTVTGTEPGQVSSR
Modification Type
Phospho (ST)
Protein - Site of Modification
439
Peptide - Site of Modification
1
Peptide Start
439
Peptide End
456
CPTAC ID
CPTAC-1048
Peptide Molecular Mass
1,953.9045
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 14.4 6 6.8 16.5 7.9 6.3 21.9 9.9 9.3 15 15 15
y6 (1+) 32.9 9.2 5.6 25.7 9 7.6 41.7 12.9 9.4 15 15 15
y4 (1+) 28.6 13.1 9.9 38.9 14.8 12.3 48.3 19.8 15.8 15 15 15
sum 9.9 4.4 5.8 13.6 6.5 5.1 16.8 7.8 7.7 15 15 15

Additional Resources and Comments