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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP4K2 GCK,RAB8IP
Sequence Length (AA) Molecular Weight (Da)
820 91556
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLRDVSLQ DPRDRFELLQ RVGAGTYGDV YKARDTVTSE LAAVKIVKLD
60 70 80 90 100
PGDDISSLQQ EITILRECRH PNVVAYIGSY LRNDRLWICM EFCGGGSLQE
110 120 130 140 150
IYHATGPLEE RQIAYVCREA LKGLHHLHSQ GKIHRDIKGA NLLLTLQGDV
160 170 180 190 200
KLADFGVSGE LTASVAKRRS FIGTPYWMAP EVAAVERKGG YNELCDVWAL
210 220 230 240 250
GITAIELGEL QPPLFHLHPM RALMLMSKSS FQPPKLRDKT RWTQNFHHFL
260 270 280 290 300
KLALTKNPKK RPTAEKLLQH PFTTQQLPRA LLTQLLDKAS DPHLGTPSPE
310 320 330 340 350
DCELETYDMF PDTIHSRGQH GPAERTPSEI QFHQVKFGAP RRKETDPLNE
360 370 380 390 400
PWEEEWTLLG KEELSGSLLQ SVQEALEERS LTIRSASEFQ ELDSPDDTMG
410 420 430 440 450
TIKRAPFLGP LPTDPPAEEP LSSPPGTLPP PPSGPNSSPL LPTAWATMKQ
460 470 480 490 500
REDPERSSCH GLPPTPKVHM GACFSKVFNG CPLRIHAAVT WIHPVTRDQF
510 520 530 540 550
LVVGAEEGIY TLNLHELHED TLEKLISHRC SWLYCVNNVL LSLSGKSTHI
560 570 580 590 600
WAHDLPGLFE QRRLQQQVPL SIPTNRLTQR IIPRRFALST KIPDTKGCLQ
610 620 630 640 650
CRVVRNPYTG ATFLLAALPT SLLLLQWYEP LQKFLLLKNF SSPLPSPAGM
660 670 680 690 700
LEPLVLDGKE LPQVCVGAEG PEGPGCRVLF HVLPLEAGLT PDILIPPEGI
710 720 730 740 750
PGSAQQVIQV DRDTILVSFE RCVRIVNMQG EPTATLAPEL TFDFPIETVV
760 770 780 790 800
CLQDSVLAFW SHGMQGRSLD TNEVTQEITD ETRIFRVLGA HRDIILESIP
810 820
TDNPEAHSNL YILTGHQSTY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-862 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K2
Peptide Sequence
IHAAVTWIHPVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
485
Peptide End
497
CPTAC ID
CPTAC-862
Peptide Molecular Mass
1,499.8361
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 6.3 4.2 5.2 5.9 4.8 6.1 8.6 6.4 8 15 15 15
b4 (1+) 15.5 6.9 7.8 15.8 7.1 9 22.1 9.9 11.9 15 15 15
b3 (1+) 7.1 7.6 5.7 9.7 7.9 5.7 12 11 8.1 15 15 15
sum 5.8 3.2 4 6.2 3.8 4.3 8.5 5 5.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-863 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K2
Peptide Modified Sequence
VFNGC[+57.0]PLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
481
Peptide - Site of Modification
5
Peptide Start
477
Peptide End
484
CPTAC ID
CPTAC-863
Peptide Molecular Mass
961.4804
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.8 4 3.8 14.1 9 6.3 17.8 9.8 7.4 15 15 15
y4 (1+) 11.2 8.7 7 9.6 8.8 5.1 14.8 12.4 8.7 15 15 15
y5 (1+) 7.5 3.5 2 7.7 3.5 4.7 10.7 4.9 5.1 15 15 15
y6 (1+) 3.5 1.9 3.5 4.9 2.1 3 6 2.8 4.6 15 15 15
y7 (1+) 7.2 3.9 6 6.3 5.1 6 9.6 6.4 8.5 15 15 15
sum 4 1.6 1.2 3.5 2.3 2 5.3 2.8 2.3 15 15 15

Additional Resources and Comments