Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP4K3 RAB8IPL1
Sequence Length (AA) Molecular Weight (Da)
894 101316
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNPGFDLSRR NPQEDFELIQ RIGSGTYGDV YKARNVNTGE LAAIKVIKLE
60 70 80 90 100
PGEDFAVVQQ EIIMMKDCKH PNIVAYFGSY LRRDKLWICM EFCGGGSLQD
110 120 130 140 150
IYHVTGPLSE LQIAYVSRET LQGLYYLHSK GKMHRDIKGA NILLTDNGHV
160 170 180 190 200
KLADFGVSAQ ITATIAKRKS FIGTPYWMAP EVAAVERKGG YNQLCDLWAV
210 220 230 240 250
GITAIELAEL QPPMFDLHPM RALFLMTKSN FQPPKLKDKM KWSNSFHHFV
260 270 280 290 300
KMALTKNPKK RPTAEKLLQH PFVTQHLTRS LAIELLDKVN NPDHSTYHDF
310 320 330 340 350
DDDDPEPLVA VPHRIHSTSR NVREEKTRSE ITFGQVKFDP PLRKETEPHH
360 370 380 390 400
ELPDSDGFLD SSEEIYYTAR SNLDLQLEYG QGHQGGYFLG ANKSLLKSVE
410 420 430 440 450
EELHQRGHVA HLEDDEGDDD ESKHSTLKAK IPPPLPPKPK SIFIPQEMHS
460 470 480 490 500
TEDENQGTIK RCPMSGSPAK PSQVPPRPPP PRLPPHKPVA LGNGMSSFQL
510 520 530 540 550
NGERDGSLCQ QQNEHRGTNL SRKEKKDVPK PISNGLPPTP KVHMGACFSK
560 570 580 590 600
VFNGCPLKIH CASSWINPDT RDQYLIFGAE EGIYTLNLNE LHETSMEQLF
610 620 630 640 650
PRRCTWLYVM NNCLLSISGK ASQLYSHNLP GLFDYARQMQ KLPVAIPAHK
660 670 680 690 700
LPDRILPRKF SVSAKIPETK WCQKCCVVRN PYTGHKYLCG ALQTSIVLLE
710 720 730 740 750
WVEPMQKFML IKHIDFPIPC PLRMFEMLVV PEQEYPLVCV GVSRGRDFNQ
760 770 780 790 800
VVRFETVNPN STSSWFTESD TPQTNVTHVT QLERDTILVC LDCCIKIVNL
810 820 830 840 850
QGRLKSSRKL SSELTFDFQI ESIVCLQDSV LAFWKHGMQG RSFRSNEVTQ
860 870 880 890 894
EISDSTRIFR LLGSDRVVVL ESRPTDNPTA NSNLYILAGH ENSY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-864 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K3
Peptide Modified Sequence
HIDFPIPC[+57.0]PLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
720
Peptide - Site of Modification
8
Peptide Start
713
Peptide End
723
CPTAC ID
CPTAC-864
Peptide Molecular Mass
1,363.7071
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.3 8.9 5.2 14.9 8.7 5.5 19.3 12.4 7.6 15 15 15
y4 (1+) 13.8 8.3 6.7 17.4 8.8 9.2 22.2 12.1 11.4 15 15 15
y5 (1+) 8.2 2.8 2.4 8 3.4 2.5 11.5 4.4 3.5 15 15 15
b4 (1+) 6.5 4.5 3.2 8.2 4.8 3.6 10.5 6.6 4.8 15 15 15
b3 (1+) 11 3.3 2.5 10.7 5.2 3.1 15.3 6.2 4 15 15 15
sum 3.1 3 1.9 4.8 3.5 1.8 5.7 4.6 2.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-865 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K3
Peptide Sequence
LLQHPFVTQHLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
267
Peptide End
279
CPTAC ID
CPTAC-865
Peptide Molecular Mass
1,588.8838
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9 6.3 5.9 9.9 8.2 7.2 13.4 10.3 9.3 15 15 15
y4 (1+) 13.2 6.5 4.5 8.7 6.2 4.1 15.8 9 6.1 15 15 15
y5 (1+) 8.4 11.6 5.3 9.2 11.2 7.6 12.5 16.1 9.3 15 15 15
y6 (1+) 10.4 5.4 6.1 9.6 6.5 5.9 14.2 8.5 8.5 15 15 15
y7 (1+) 14 5.3 3.1 16.3 6.5 4.3 21.5 8.4 5.3 15 15 15
y8 (1+) 13 7.6 8.4 13.1 7.2 10.3 18.5 10.5 13.3 15 15 15
sum 6 3.2 2.6 5.5 3.5 2.5 8.1 4.7 3.6 15 15 15

Additional Resources and Comments