MAPK13, CPTAC-876 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK13	PRKM13, SAPK4

Sequence Length (AA)	Molecular Weight (Da)
365	42090

Protein Name
Mitogen-activated protein kinase 13

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLIRKKGFY	KQDVNKTAWE	LPKTYVSPTH	VGSGAYGSVC	SAIDKRSGEK
60	70	80	90	100
VAIKKLSRPF	QSEIFAKRAY	RELLLLKHMQ	HENVIGLLDV	FTPASSLRNF
110	120	130	140	150
YDFYLVMPFM	QTDLQKIMGM	EFSEEKIQYL	VYQMLKGLKY	IHSAGVVHRD
160	170	180	190	200
LKPGNLAVNE	DCELKILDFG	LARHADAEMT	GYVVTRWYRA	PEVILSWMHY
210	220	230	240	250
NQTVDIWSVG	CIMAEMLTGK	TLFKGKDYLD	QLTQILKVTG	VPGTEFVQKL
260	270	280	290	300
NDKAAKSYIQ	SLPQTPRKDF	TQLFPRASPQ	AADLLEKMLE	LDVDKRLTAA
310	320	330	340	350
QALTHPFFEP	FRDPEEETEA	QQPFDDSLEH	EKLTVDEWKQ	HIYKEIVNFS
360	365
PIARKDSRRR	SGMKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-876 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK13
Peptide Sequence: EIVNFSPIAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 345
Peptide End: 354

CPTAC ID: CPTAC-876
Peptide Molecular Mass: 1,144.6241
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	8.5	5.9	4	9.1	4.5	4.5	12.5	7.4	6	15	15	15
y5 (1+)	6.2	4.2	7.6	6.3	4.9	6.4	8.8	6.5	9.9	15	15	15
y6 (1+)	8.1	4.8	6.4	7	6.2	6.2	10.7	7.8	8.9	15	15	15
y7 (1+)	6.5	5.2	3.6	6.9	4	3.1	9.5	6.6	4.8	15	15	15
y8 (1+)	8.6	6.4	5.4	8.8	7.3	6.1	12.3	9.7	8.1	15	15	15
sum	2.3	2.2	2.2	3.1	2.6	2.3	3.9	3.4	3.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-877 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK13
Peptide Sequence: SYIQSLPQTPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 257
Peptide End: 267

CPTAC ID: CPTAC-877
Peptide Molecular Mass: 1,288.6776
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend