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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK13 PRKM13, SAPK4
Sequence Length (AA) Molecular Weight (Da)
365 42090
Protein Name
Mitogen-activated protein kinase 13
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK
60 70 80 90 100
VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF
110 120 130 140 150
YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD
160 170 180 190 200
LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY
210 220 230 240 250
NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL
260 270 280 290 300
NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA
310 320 330 340 350
QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS
360 365
PIARKDSRRR SGMKL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-876 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK13
Peptide Sequence
EIVNFSPIAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
345
Peptide End
354
CPTAC ID
CPTAC-876
Peptide Molecular Mass
1,144.6241
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 8.5 5.9 4 9.1 4.5 4.5 12.5 7.4 6 15 15 15
y5 (1+) 6.2 4.2 7.6 6.3 4.9 6.4 8.8 6.5 9.9 15 15 15
y6 (1+) 8.1 4.8 6.4 7 6.2 6.2 10.7 7.8 8.9 15 15 15
y7 (1+) 6.5 5.2 3.6 6.9 4 3.1 9.5 6.6 4.8 15 15 15
y8 (1+) 8.6 6.4 5.4 8.8 7.3 6.1 12.3 9.7 8.1 15 15 15
sum 2.3 2.2 2.2 3.1 2.6 2.3 3.9 3.4 3.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-877 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK13
Peptide Sequence
SYIQSLPQTPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
257
Peptide End
267
CPTAC ID
CPTAC-877
Peptide Molecular Mass
1,288.6776
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.9 3.7 3.9 6.8 3.8 5 9.7 5.3 6.3 15 15 15
y6 (1+) 16.1 12.1 8.3 14.6 15.9 9.9 21.7 20 12.9 15 15 15
y7 (1+) 9.9 3.6 4.4 9.8 3.2 4.8 13.9 4.8 6.5 15 15 15
y8 (1+) 12.6 5.1 5.8 15.6 6.8 6.7 20.1 8.5 8.9 15 15 15
y9 (1+) 18.5 7.6 8.3 18.2 7.8 8.4 26 10.9 11.8 15 15 15
sum 5.9 2.2 1.8 6.4 2.6 2.5 8.7 3.4 3.1 15 15 15

Additional Resources and Comments