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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK4 ERK4,PRKM4
Sequence Length (AA) Molecular Weight (Da)
587 65922
Protein Name
Mitogen-activated protein kinase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEKGDCIAS VYGYDLGGRF VDFQPLGFGV NGLVLSAVDS RACRKVAVKK
60 70 80 90 100
IALSDARSMK HALREIKIIR RLDHDNIVKV YEVLGPKGTD LQGELFKFSV
110 120 130 140 150
AYIVQEYMET DLARLLEQGT LAEEHAKLFM YQLLRGLKYI HSANVLHRDL
160 170 180 190 200
KPANIFISTE DLVLKIGDFG LARIVDQHYS HKGYLSEGLV TKWYRSPRLL
210 220 230 240 250
LSPNNYTKAI DMWAAGCILA EMLTGRMLFA GAHELEQMQL ILETIPVIRE
260 270 280 290 300
EDKDELLRVM PSFVSSTWEV KRPLRKLLPE VNSEAIDFLE KILTFNPMDR
310 320 330 340 350
LTAEMGLQHP YMSPYSCPED EPTSQHPFRI EDEIDDIVLM AANQSQLSNW
360 370 380 390 400
DTCSSRYPVS LSSDLEWRPD RCQDASEVQR DPRAGSAPLA EDVQVDPRKD
410 420 430 440 450
SHSSSERFLE QSHSSMERAF EADYGRSCDY KVGSPSYLDK LLWRDNKPHH
460 470 480 490 500
YSEPKLILDL SHWKQAAGAP PTATGLADTG AREDEPASLF LEIAQWVKST
510 520 530 540 550
QGGPEHASPP ADDPERRLSA SPPGRPAPVD GGASPQFDLD VFISRALKLC
560 570 580 587
TKPEDLPDNK LGDLNGACIP EHPGDLVQTE AFSKERW

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-883 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK4
Peptide Sequence
GYLSEGLVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
192
CPTAC ID
CPTAC-883
Peptide Molecular Mass
1,065.5706
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.1 8.2 5.7 12.5 8.9 6.4 15.5 12.1 8.6 15 15 15
y5 (1+) 8.1 7.4 7.5 7.9 7.7 5.4 11.3 10.7 9.2 15 15 15
y6 (1+) 13.9 4.5 7.6 11.6 5.2 8.4 18.1 6.9 11.3 15 15 15
y7 (1+) 5.8 3.7 3.1 4.9 5.2 2.8 7.6 6.4 4.2 15 15 15
y8 (1+) 6.6 3.3 5 6.6 4.6 4.8 9.3 5.7 6.9 15 15 15
sum 2.8 3.1 2.9 3.3 4.1 2.8 4.3 5.1 4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-884 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK4
Peptide Sequence
VGSPSYLDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
432
Peptide End
440
CPTAC ID
CPTAC-884
Peptide Molecular Mass
964.4866
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.1 4.7 4.7 9.2 5.7 5 13.7 7.4 6.9 15 15 15
y4 (1+) 7.4 7.8 4.1 9.1 7.5 4.1 11.7 10.8 5.8 15 15 15
y5 (1+) 6.9 3.3 3.1 7.3 3 4 10 4.5 5.1 15 15 15
y6 (1+) 7.8 4.1 2.6 7.8 3.6 3.7 11 5.5 4.5 15 15 15
y7 (1+) 7.5 8.9 5.2 7.4 8.3 5.3 10.5 12.2 7.4 15 15 15
y8 (1+) 7.2 4.7 4.8 5.2 3.2 4.4 8.9 5.7 6.5 15 15 15
b3 (1+) 21.9 5.9 10.6 24 12.9 10.9 32.5 14.2 15.2 15 15 15
sum 2.6 1.9 1.8 2.3 2 2.2 3.5 2.8 2.8 15 15 15

Additional Resources and Comments