MAPK4, CPTAC-884 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK4	ERK4, PRKM4

Sequence Length (AA)	Molecular Weight (Da)
587	65922

Protein Name
Mitogen-activated protein kinase 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAEKGDCIAS	VYGYDLGGRF	VDFQPLGFGV	NGLVLSAVDS	RACRKVAVKK
60	70	80	90	100
IALSDARSMK	HALREIKIIR	RLDHDNIVKV	YEVLGPKGTD	LQGELFKFSV
110	120	130	140	150
AYIVQEYMET	DLARLLEQGT	LAEEHAKLFM	YQLLRGLKYI	HSANVLHRDL
160	170	180	190	200
KPANIFISTE	DLVLKIGDFG	LARIVDQHYS	HKGYLSEGLV	TKWYRSPRLL
210	220	230	240	250
LSPNNYTKAI	DMWAAGCILA	EMLTGRMLFA	GAHELEQMQL	ILETIPVIRE
260	270	280	290	300
EDKDELLRVM	PSFVSSTWEV	KRPLRKLLPE	VNSEAIDFLE	KILTFNPMDR
310	320	330	340	350
LTAEMGLQHP	YMSPYSCPED	EPTSQHPFRI	EDEIDDIVLM	AANQSQLSNW
360	370	380	390	400
DTCSSRYPVS	LSSDLEWRPD	RCQDASEVQR	DPRAGSAPLA	EDVQVDPRKD
410	420	430	440	450
SHSSSERFLE	QSHSSMERAF	EADYGRSCDY	KVGSPSYLDK	LLWRDNKPHH
460	470	480	490	500
YSEPKLILDL	SHWKQAAGAP	PTATGLADTG	AREDEPASLF	LEIAQWVKST
510	520	530	540	550
QGGPEHASPP	ADDPERRLSA	SPPGRPAPVD	GGASPQFDLD	VFISRALKLC
560	570	580	587
TKPEDLPDNK	LGDLNGACIP	EHPGDLVQTE	AFSKERW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-883 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK4
Peptide Sequence: GYLSEGLVTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 183
Peptide End: 192

CPTAC ID: CPTAC-883
Peptide Molecular Mass: 1,065.5706
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	9.1	8.2	5.7	12.5	8.9	6.4	15.5	12.1	8.6	15	15	15
y5 (1+)	8.1	7.4	7.5	7.9	7.7	5.4	11.3	10.7	9.2	15	15	15
y6 (1+)	13.9	4.5	7.6	11.6	5.2	8.4	18.1	6.9	11.3	15	15	15
y7 (1+)	5.8	3.7	3.1	4.9	5.2	2.8	7.6	6.4	4.2	15	15	15
y8 (1+)	6.6	3.3	5	6.6	4.6	4.8	9.3	5.7	6.9	15	15	15
sum	2.8	3.1	2.9	3.3	4.1	2.8	4.3	5.1	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-884 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK4
Peptide Sequence: VGSPSYLDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 432
Peptide End: 440

CPTAC ID: CPTAC-884
Peptide Molecular Mass: 964.4866
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend