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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAPK6

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK6 ERK3, PRKM6
Sequence Length (AA) Molecular Weight (Da)
721 82681
Protein Name
Mitogen-activated protein kinase 6
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEKFESLMN IHGFDLGSRY MDLKPLGCGG NGLVFSAVDN DCDKRVAIKK
60 70 80 90 100
IVLTDPQSVK HALREIKIIR RLDHDNIVKV FEILGPSGSQ LTDDVGSLTE
110 120 130 140 150
LNSVYIVQEY METDLANVLE QGPLLEEHAR LFMYQLLRGL KYIHSANVLH
160 170 180 190 200
RDLKPANLFI NTEDLVLKIG DFGLARIMDP HYSHKGHLSE GLVTKWYRSP
210 220 230 240 250
RLLLSPNNYT KAIDMWAAGC IFAEMLTGKT LFAGAHELEQ MQLILESIPV
260 270 280 290 300
VHEEDRQELL SVIPVYIRND MTEPHKPLTQ LLPGISREAL DFLEQILTFS
310 320 330 340 350
PMDRLTAEEA LSHPYMSIYS FPMDEPISSH PFHIEDEVDD ILLMDETHSH
360 370 380 390 400
IYNWERYHDC QFSEHDWPVH NNFDIDEVQL DPRALSDVTD EEEVQVDPRK
410 420 430 440 450
YLDGDREKYL EDPAFDTNYS TEPCWQYSDH HENKYCDLEC SHTCNYKTRS
460 470 480 490 500
SSYLDNLVWR ESEVNHYYEP KLIIDLSNWK EQSKEKSDKK GKSKCERNGL
510 520 530 540 550
VKAQIALEEA SQQLAGKERE KNQGFDFDSF IAGTIQLSSQ HEPTDVVDKL
560 570 580 590 600
NDLNSSVSQL ELKSLISKSV SQEKQEKGMA NLAQLEALYQ SSWDSQFVSG
610 620 630 640 650
GEDCFFINQF CEVRKDEQVE KENTYTSYLD KFFSRKEDTE MLETEPVEDG
660 670 680 690 700
KLGERGHEEG FLNNSGEFLF NKQLESIGIP QFHSPVGSPL KSIQATLTPS
710 720 721
AMKSSPQIPH QTYSSILKHL N

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-885 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK6
Peptide Sequence
SSPQIPHQTYSSILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
704
Peptide End
718
CPTAC ID
CPTAC-885
Peptide Molecular Mass
1,684.8784
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 12.8 12.7 4.9 19.1 11.5 9.9 23 17.1 11 15 15 15
y6 (1+) 23.9 10.1 10.9 22 10.1 9 32.5 14.3 14.1 15 15 15
y7 (1+) 13.4 4.6 7.5 18.2 5 8.3 22.6 6.8 11.2 15 15 15
y8 (1+) 16.8 5.9 8.8 18.9 5.2 9.2 25.3 7.9 12.7 15 15 15
y10 (1+) 8 7.2 5.6 7.6 6.8 5.5 11 9.9 7.8 15 15 15
b10 (1+) 19 12 9.6 20.2 14.2 9.9 27.7 18.6 13.8 15 15 15
sum 6.3 3.7 3 8.3 3.7 2.6 10.4 5.2 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-886 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK6
Peptide Sequence
SSSYLDNLVWR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
450
Peptide End
460
CPTAC ID
CPTAC-886
Peptide Molecular Mass
1,338.6568
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.8 11 12.2 14.9 14.3 12.4 19.6 18 17.4 15 15 15
y4 (1+) 23.3 12.6 11.7 23.8 12.2 12.3 33.3 17.5 17 15 15 15
y5 (1+) 9.6 9.5 4 10.5 9.3 5.3 14.2 13.3 6.6 15 15 15
y6 (1+) 6.8 5.1 4.4 7.6 6.2 3.1 10.2 8 5.4 15 15 15
y7 (1+) 14.2 4.6 5.3 13.9 5.1 5.1 19.9 6.9 7.4 15 15 15
y8 (1+) 14.4 11.4 9.9 14.4 13.2 11.8 20.4 17.4 15.4 15 15 15
y9 (1+) 22 7.1 10.3 24.5 12 9.4 32.9 13.9 13.9 15 15 15
sum 5.5 3.7 3.2 5.8 4 3.6 8 5.4 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments