MLKL, CPTAC-896 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MLKL	N/A

Sequence Length (AA)	Molecular Weight (Da)
471	54479

Protein Name
Mixed lineage kinase domain-like protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MENLKHIITL	GQVIHKRCEE	MKYCKKQCRR	LGHRVLGLIK	PLEMLQDQGK
60	70	80	90	100
RSVPSEKLTT	AMNRFKAALE	EANGEIEKFS	NRSNICRFLT	ASQDKILFKD
110	120	130	140	150
VNRKLSDVWK	ELSLLLQVEQ	RMPVSPISQG	ASWAQEDQQD	ADEDRRAFQM
160	170	180	190	200
LRRDNEKIEA	SLRRLEINMK	EIKETLRQYL	PPKCMQEIPQ	EQIKEIKKEQ
210	220	230	240	250
LSGSPWILLR	ENEVSTLYKG	EYHRAPVAIK	VFKKLQAGSI	AIVRQTFNKE
260	270	280	290	300
IKTMKKFESP	NILRIFGICI	DETVTPPQFS	IVMEYCELGT	LRELLDREKD
310	320	330	340	350
LTLGKRMVLV	LGAARGLYRL	HHSEAPELHG	KIRSSNFLVT	QGYQVKLAGF
360	370	380	390	400
ELRKTQTSMS	LGTTREKTDR	VKSTAYLSPQ	ELEDVFYQYD	VKSEIYSFGI
410	420	430	440	450
VLWEIATGDI	PFQGCNSEKI	RKLVAVKRQQ	EPLGEDCPSE	LREIIDECRA
460	470	471
HDPSVRPSVD	EILKKLSTFS	K

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-895 Collapse assay details

Data source: Panorama

Official Gene Symbol: MLKL
Peptide Sequence: LAGFELR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 347
Peptide End: 353

CPTAC ID: CPTAC-895
Peptide Molecular Mass: 804.4494
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	14.2	12.4	7.3	13.7	14.1	8.4	19.7	18.8	11.1	15	15	15
y4 (1+)	15.4	11.8	13.5	16.2	10	13.8	22.4	15.5	19.3	15	15	15
y5 (1+)	6.9	3.9	4.5	7	5.3	4.8	9.8	6.6	6.6	15	15	15
y6 (1+)	5.6	3.6	4.7	8	5.7	5	9.8	6.7	6.9	15	15	15
sum	5.1	3	3.7	6.3	4.6	3.2	8.1	5.5	4.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-896 Collapse assay details

Data source: Panorama

Official Gene Symbol: MLKL
Peptide Sequence: LQAGSIAIVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 235
Peptide End: 244

CPTAC ID: CPTAC-896
Peptide Molecular Mass: 1,026.6186
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend