Overview Data source: UniProt

Official Gene Symbol Other Aliases
MLKL N/A
Sequence Length (AA) Molecular Weight (Da)
471 54479
Protein Name
Mixed lineage kinase domain-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENLKHIITL GQVIHKRCEE MKYCKKQCRR LGHRVLGLIK PLEMLQDQGK
60 70 80 90 100
RSVPSEKLTT AMNRFKAALE EANGEIEKFS NRSNICRFLT ASQDKILFKD
110 120 130 140 150
VNRKLSDVWK ELSLLLQVEQ RMPVSPISQG ASWAQEDQQD ADEDRRAFQM
160 170 180 190 200
LRRDNEKIEA SLRRLEINMK EIKETLRQYL PPKCMQEIPQ EQIKEIKKEQ
210 220 230 240 250
LSGSPWILLR ENEVSTLYKG EYHRAPVAIK VFKKLQAGSI AIVRQTFNKE
260 270 280 290 300
IKTMKKFESP NILRIFGICI DETVTPPQFS IVMEYCELGT LRELLDREKD
310 320 330 340 350
LTLGKRMVLV LGAARGLYRL HHSEAPELHG KIRSSNFLVT QGYQVKLAGF
360 370 380 390 400
ELRKTQTSMS LGTTREKTDR VKSTAYLSPQ ELEDVFYQYD VKSEIYSFGI
410 420 430 440 450
VLWEIATGDI PFQGCNSEKI RKLVAVKRQQ EPLGEDCPSE LREIIDECRA
460 470 471
HDPSVRPSVD EILKKLSTFS K

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-895 Collapse assay details

Data source: Panorama

Official Gene Symbol
MLKL
Peptide Sequence
LAGFELR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
347
Peptide End
353
CPTAC ID
CPTAC-895
Peptide Molecular Mass
804.4494
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.2 12.4 7.3 13.7 14.1 8.4 19.7 18.8 11.1 15 15 15
y4 (1+) 15.4 11.8 13.5 16.2 10 13.8 22.4 15.5 19.3 15 15 15
y5 (1+) 6.9 3.9 4.5 7 5.3 4.8 9.8 6.6 6.6 15 15 15
y6 (1+) 5.6 3.6 4.7 8 5.7 5 9.8 6.7 6.9 15 15 15
sum 5.1 3 3.7 6.3 4.6 3.2 8.1 5.5 4.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-896 Collapse assay details

Data source: Panorama

Official Gene Symbol
MLKL
Peptide Sequence
LQAGSIAIVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
235
Peptide End
244
CPTAC ID
CPTAC-896
Peptide Molecular Mass
1,026.6186
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 20.6 11.5 14 16.7 11 16 26.5 15.9 21.3 15 15 15
y4 (1+) 8.4 4 6.3 9.1 4.8 6.5 12.4 6.2 9.1 15 15 15
y5 (1+) 12 8.2 9.1 14.8 10.8 10.8 19.1 13.6 14.1 15 15 15
y7 (1+) 11.8 5.9 4.1 11.7 5.1 3.8 16.6 7.8 5.6 15 15 15
y8 (1+) 3.5 2.6 2.4 4.9 3.1 2.8 6 4 3.7 15 15 15
sum 4.4 1.3 2.1 4.5 2.1 2.1 6.3 2.5 3 15 15 15

Additional Resources and Comments