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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAPK9

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK9 JNK2, PRKM9, SAPK1A
Sequence Length (AA) Molecular Weight (Da)
424 48139
Protein Name
Mitogen-activated protein kinase 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI
60 70 80 90 100
NVAVKKLSRP FQNQTHAKRA YRELVLLKCV NHKNIISLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDIWS VGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK
260 270 280 290 300
KLQPTVRNYV ENRPKYPGIK FEELFPDWIF PSESERDKIK TSQARDLLSK
310 320 330 340 350
MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIE
360 370 380 390 400
EWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS
410 420 424
TEQTLASDTD SSLDASTGPL EGCR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3146 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK9
Peptide Sequence
EHAIEEWK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
346
Peptide End
353
CPTAC ID
CPTAC-3146
Peptide Molecular Mass
1,040.4927
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 7.6 6.6 11.6 6.2 7.1 11.5 9.8 9.7 16.3 15 15 15
y6 (1+) 3.8 7.1 8.2 5.7 7.7 10.2 6.9 10.5 13.1 15 15 15
y4 (1+) 11.4 7.8 12 12.9 9.9 13.8 17.2 12.6 18.3 15 15 15
sum 3.2 5.7 8.4 4 6.2 9.5 5.1 8.4 12.7 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-893 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK9
Peptide Sequence
FEELFPDWIFPSESER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
271
Peptide End
286
CPTAC ID
CPTAC-893
Peptide Molecular Mass
2,026.9313
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 74.4 40 19.9 69.6 48.3 27.7 101.9 62.7 34.1 10 15 15
y4 (1+) 105.6 42 27.9 113.5 45.1 42.3 155 61.6 50.7 11 15 15
y5 (1+) 24.8 15.2 21.7 48.3 27.2 22.9 54.3 31.2 31.5 10 15 15
y6 (1+) 37.8 17 8.8 102.5 21.1 10.5 109.2 27.1 13.7 13 15 15
y7 (1+) 35.9 15.8 12.3 74.9 15.7 14 83.1 22.3 18.6 12 15 15
y8 (1+) 75 45.4 37.8 87.4 47.4 42.5 115.2 65.6 56.9 10 12 15
y9 (1+) 77.6 58.4 42.6 95.2 66.2 33.1 122.8 88.3 53.9 8 15 15
y10 (1+) 110.9 61.9 33.8 145.6 59.1 49.8 183 85.6 60.2 9 11 13
y11 (1+) 60.7 62.4 43.8 157.3 141.6 48.4 168.6 154.7 65.3 6 11 14
b11 (1+) NA NA NA NA NA NA NA NA NA 0 1 1
b10 (1+) NA 94.3 NA NA 0.2 NA NA 94.3 NA 3 4 3
b9 (1+) 45.7 44.2 77.3 173.2 38.5 86.4 179.1 58.6 115.9 5 7 13
b8 (1+) 44.7 58.8 29.8 81 54.3 27.3 92.5 80 40.4 9 15 13
b7 (1+) 82.8 51.5 75.3 113.7 51.4 79.8 140.7 72.8 109.7 11 12 14
b6 (1+) 141.3 72.8 73.8 36.8 77.3 71.4 146 106.2 102.7 9 9 12
b5 (1+) 77.7 60.1 56 62.5 67.6 79.6 99.7 90.5 97.3 10 14 15
b4 (1+) 64.9 38.2 23.8 138.6 38.6 30 153 54.3 38.3 11 15 15
b3 (1+) 73.9 54.7 17.4 71.7 73.7 20.6 103 91.8 27 13 15 15
sum 50 6.4 4 88.4 11 5.2 101.6 12.7 6.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for CPTAC-894 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK9
Peptide Sequence
VIEQLGTPSAEFMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
237
Peptide End
250
CPTAC ID
CPTAC-894
Peptide Molecular Mass
1,548.7858
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 5.7 6.4 7.3 8.4 6.2 6.7 10.2 8.9 9.9 15 15 15
y4 (1+) 18.8 10.2 6.4 17.9 9.1 5.8 26 13.7 8.6 15 15 15
y5 (1+) 16.9 11.6 3.5 15.3 11.4 5.5 22.8 16.3 6.5 15 15 15
y6 (1+) 15.4 8.4 6.2 14 7.6 6.4 20.8 11.3 8.9 15 15 15
y7 (1+) 5.4 3.3 1.9 4.7 4.7 2.2 7.2 5.7 2.9 15 15 15
y8 (1+) 20.5 8.2 4.2 19.9 6.9 5.3 28.6 10.7 6.8 15 15 15
y9 (1+) 5.2 3.5 2.2 8.7 4 2.2 10.1 5.3 3.1 15 15 15
y10 (1+) 12 10.4 4.5 12.3 11.2 4.8 17.2 15.3 6.6 15 15 15
b4 (1+) 21.7 10.9 8.6 21.5 9.6 12.1 30.5 14.5 14.8 15 15 15
b3 (1+) 26.6 4.7 4.5 25.1 6.5 4.6 36.6 8 6.4 15 15 15
sum 3.6 2.6 1.4 3.7 2.4 1.8 5.2 3.5 2.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments