Overview Data source: UniProt

Official Gene Symbol Other Aliases
RIPK3 RIP3
Sequence Length (AA) Molecular Weight (Da)
518 56887
Protein Name
Receptor-interacting serine/threonine-protein kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSCVKLWPSG APAPLVSIEE LENQELVGKG GFGTVFRAQH RKWGYDVAVK
60 70 80 90 100
IVNSKAISRE VKAMASLDNE FVLRLEGVIE KVNWDQDPKP ALVTKFMENG
110 120 130 140 150
SLSGLLQSQC PRPWPLLCRL LKEVVLGMFY LHDQNPVLLH RDLKPSNVLL
160 170 180 190 200
DPELHVKLAD FGLSTFQGGS QSGTGSGEPG GTLGYLAPEL FVNVNRKAST
210 220 230 240 250
ASDVYSFGIL MWAVLAGREV ELPTEPSLVY EAVCNRQNRP SLAELPQAGP
260 270 280 290 300
ETPGLEGLKE LMQLCWSSEP KDRPSFQECL PKTDEVFQMV ENNMNAAVST
310 320 330 340 350
VKDFLSQLRS SNRRFSIPES GQGGTEMDGF RRTIENQHSR NDVMVSEWLN
360 370 380 390 400
KLNLEEPPSS VPKKCPSLTK RSRAQEEQVP QAWTAGTSSD SMAQPPQTPE
410 420 430 440 450
TSTFRNQMPS PTSTGTPSPG PRGNQGAERQ GMNWSCRTPE PNPVTGRPLV
460 470 480 490 500
NIYNCSGVQV GDNNYLTMQQ TTALPTWGLA PSGKGRGLQH PPPVGSQEGP
510 518
KDPEAWSRPQ GWYNHSGK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-903 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK3
Peptide Sequence
DLKPSNVLLDPELHVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
142
Peptide End
157
CPTAC ID
CPTAC-903
Peptide Molecular Mass
1,816.0095
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.7 4 6.7 9.5 4 6.4 15.1 5.7 9.3 15 15 15
y6 (1+) 9.9 5.3 2.7 9.9 5 3.2 14 7.3 4.2 15 15 15
b6 (1+) 10.3 8.7 3.7 12.6 11.4 4.7 16.3 14.3 6 15 15 15
sum 6.7 4.5 2.3 7.2 4.3 2.4 9.8 6.2 3.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-904 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK3
Peptide Sequence
VNWDQDPKPALVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
82
Peptide End
95
CPTAC ID
CPTAC-904
Peptide Molecular Mass
1,609.8464
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.7 9.8 6.9 18 10.9 10.2 22 14.7 12.3 15 15 15
y5 (1+) 29.8 9.3 8.2 27.1 11.9 8.5 40.3 15.1 11.8 15 15 15
y6 (1+) 4.1 4 3 6.7 4.2 5.3 7.9 5.8 6.1 15 15 15
y7 (1+) 21.7 13.4 11 22.6 10.3 11.3 31.3 16.9 15.8 15 15 15
y8 (1+) 10 4.9 5.1 11.5 6.3 6.6 15.2 8 8.3 15 15 15
sum 3.6 3.6 2.4 6.3 3.6 4.3 7.3 5.1 4.9 15 15 15

Additional Resources and Comments