RIPK3, CPTAC-904 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIPK3	RIP3

Sequence Length (AA)	Molecular Weight (Da)
518	56887

Protein Name
Receptor-interacting serine/threonine-protein kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSCVKLWPSG	APAPLVSIEE	LENQELVGKG	GFGTVFRAQH	RKWGYDVAVK
60	70	80	90	100
IVNSKAISRE	VKAMASLDNE	FVLRLEGVIE	KVNWDQDPKP	ALVTKFMENG
110	120	130	140	150
SLSGLLQSQC	PRPWPLLCRL	LKEVVLGMFY	LHDQNPVLLH	RDLKPSNVLL
160	170	180	190	200
DPELHVKLAD	FGLSTFQGGS	QSGTGSGEPG	GTLGYLAPEL	FVNVNRKAST
210	220	230	240	250
ASDVYSFGIL	MWAVLAGREV	ELPTEPSLVY	EAVCNRQNRP	SLAELPQAGP
260	270	280	290	300
ETPGLEGLKE	LMQLCWSSEP	KDRPSFQECL	PKTDEVFQMV	ENNMNAAVST
310	320	330	340	350
VKDFLSQLRS	SNRRFSIPES	GQGGTEMDGF	RRTIENQHSR	NDVMVSEWLN
360	370	380	390	400
KLNLEEPPSS	VPKKCPSLTK	RSRAQEEQVP	QAWTAGTSSD	SMAQPPQTPE
410	420	430	440	450
TSTFRNQMPS	PTSTGTPSPG	PRGNQGAERQ	GMNWSCRTPE	PNPVTGRPLV
460	470	480	490	500
NIYNCSGVQV	GDNNYLTMQQ	TTALPTWGLA	PSGKGRGLQH	PPPVGSQEGP
510	518
KDPEAWSRPQ	GWYNHSGK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-903 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIPK3
Peptide Sequence: DLKPSNVLLDPELHVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 142
Peptide End: 157

CPTAC ID: CPTAC-903
Peptide Molecular Mass: 1,816.0095
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	11.7	4	6.7	9.5	4	6.4	15.1	5.7	9.3	15	15	15
y6 (1+)	9.9	5.3	2.7	9.9	5	3.2	14	7.3	4.2	15	15	15
b6 (1+)	10.3	8.7	3.7	12.6	11.4	4.7	16.3	14.3	6	15	15	15
sum	6.7	4.5	2.3	7.2	4.3	2.4	9.8	6.2	3.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-904 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIPK3
Peptide Sequence: VNWDQDPKPALVTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 82
Peptide End: 95

CPTAC ID: CPTAC-904
Peptide Molecular Mass: 1,609.8464
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend