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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SRC SRC1
Sequence Length (AA) Molecular Weight (Da)
536 59835
Protein Name
Proto-oncogene tyrosine-protein kinase Src
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP
60 70 80 90 100
SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE
110 120 130 140 150
TDLSFKKGER LQIVNNTEGD WWLAHSLSTG QTGYIPSNYV APSDSIQAEE
160 170 180 190 200
WYFGKITRRE SERLLLNAEN PRGTFLVRES ETTKGAYCLS VSDFDNAKGL
210 220 230 240 250
NVKHYKIRKL DSGGFYITSR TQFNSLQQLV AYYSKHADGL CHRLTTVCPT
260 270 280 290 300
SKPQTQGLAK DAWEIPRESL RLEVKLGQGC FGEVWMGTWN GTTRVAIKTL
310 320 330 340 350
KPGTMSPEAF LQEAQVMKKL RHEKLVQLYA VVSEEPIYIV TEYMSKGSLL
360 370 380 390 400
DFLKGETGKY LRLPQLVDMA AQIASGMAYV ERMNYVHRDL RAANILVGEN
410 420 430 440 450
LVCKVADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS
460 470 480 490 500
FGILLTELTT KGRVPYPGMV NREVLDQVER GYRMPCPPEC PESLHDLMCQ
510 520 530 536
CWRKEPEERP TFEYLQAFLE DYFTSTEPQY QPGENL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-905 Collapse assay details

Data source: Panorama

Official Gene Symbol
SRC
Peptide Modified Sequence
LIEDNEY[+80.0]TAR
Modification Type
Phospho (Y)
Protein - Site of Modification
419
Peptide - Site of Modification
7
Peptide Start
388
Peptide End
397
CPTAC ID
CPTAC-905
Peptide Molecular Mass
1,302.5493
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 25.3 11.9 11.2 31.4 14.5 15.2 40.3 18.8 18.9 15 15 15
y4 (1+) 14.3 11.6 8.5 14.5 14.4 7.6 20.4 18.5 11.4 15 15 15
y5 (1+) 21.1 10.3 8.4 23.6 11.5 10.9 31.7 15.4 13.8 15 15 15
y6 (1+) 6.5 6.9 7.7 11.6 9 9.6 13.3 11.3 12.3 15 15 15
y7 (1+) 14.4 8.1 7.4 12.6 8.3 9.9 19.1 11.6 12.4 15 15 15
y8 (1+) 12.9 7.1 7.1 14.9 8.6 8 19.7 11.2 10.7 15 15 15
sum 3.4 5.9 4.6 6 7.2 6 6.9 9.3 7.6 15 15 15

Additional Resources and Comments