TAB1, CPTAC-907 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TAB1	MAP3K7IP1

Sequence Length (AA)	Molecular Weight (Da)
504	54644

Protein Name
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAQRRSLLQ	SEQQPSWTDD	LPLCHLSGVG	SASNRSYSAD	GKGTESHPPE
60	70	80	90	100
DSWLKFRSEN	NCFLYGVFNG	YDGNRVTNFV	AQRLSAELLL	GQLNAEHAEA
110	120	130	140	150
DVRRVLLQAF	DVVERSFLES	IDDALAEKAS	LQSQLPEGVP	QHQLPPQYQK
160	170	180	190	200
ILERLKTLER	EISGGAMAVV	AVLLNNKLYV	ANVGTNRALL	CKSTVDGLQV
210	220	230	240	250
TQLNVDHTTE	NEDELFRLSQ	LGLDAGKIKQ	VGIICGQEST	RRIGDYKVKY
260	270	280	290	300
GYTDIDLLSA	AKSKPIIAEP	EIHGAQPLDG	VTGFLVLMSE	GLYKALEAAH
310	320	330	340	350
GPGQANQEIA	AMIDTEFAKQ	TSLDAVAQAV	VDRVKRIHSD	TFASGGERAR
360	370	380	390	400
FCPRHEDMTL	LVRNFGYPLG	EMSQPTPSPA	PAAGGRVYPV	SVPYSSAQST
410	420	430	440	450
SKTSVTLSLV	MPSQGQMVNG	AHSASTLDEA	TPTLTNQSPT	LTLQSTNTHT
460	470	480	490	500
QSSSSSSDGG	LFRSRPAHSL	PPGEDGRVEP	YVDFAEFYRL	WSVDHGEQSV
504
VTAP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-906 Collapse assay details

Data source: Panorama

Official Gene Symbol: TAB1
Peptide Sequence: GTESHPPEDSWLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 43
Peptide End: 55

CPTAC ID: CPTAC-906
Peptide Molecular Mass: 1,481.6787
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	37	22	18.8	35.1	24	27.9	51	32.6	33.6	15	15	15
y4 (1+)	29.5	9.1	6.1	28.9	8.3	7.5	41.3	12.3	9.7	15	15	15
y7 (1+)	12.2	3.3	4.1	15.6	3.7	5.6	19.8	5	6.9	15	15	15
y8 (1+)	10.2	6.6	5.5	9.9	6.9	6	14.2	9.5	8.1	15	15	15
sum	7.2	5.2	3.9	7.7	5.6	4.1	10.5	7.6	5.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-907 Collapse assay details

Data source: Panorama

Official Gene Symbol: TAB1
Peptide Modified Sequence: QVGIIC[+57.0]GQESTR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 235
Peptide - Site of Modification: 6
Peptide Start: 230
Peptide End: 241

CPTAC ID: CPTAC-907
Peptide Molecular Mass: 1,346.6612
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend