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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

LCK

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LCK N/A
Sequence Length (AA) Molecular Weight (Da)
509 58001
Protein Name
Tyrosine-protein kinase Lck
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCGCSSHPE DDWMENIDVC ENCHYPIVPL DGKGTLLIRN GSEVRDPLVT
60 70 80 90 100
YEGSNPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA
110 120 130 140 150
QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS
160 170 180 190 200
FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP
210 220 230 240 250
GLHELVRHYT NASDGLCTRL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER
260 270 280 290 300
LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHQRL
310 320 330 340 350
VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLTINK LLDMAAQIAE
360 370 380 390 400
GMAFIEERNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA
410 420 430 440 450
KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI
460 470 480 490 500
QNLERGYRMV RPDNCPEELY QLMRLCWKER PEDRPTFDYL RSVLEDFFTA
509
TEGQYQPQP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-929 Collapse assay details

Data source: Panorama

Official Gene Symbol
LCK
Peptide Modified Sequence
NLDNGGFY[+80.0]ISPR
Modification Type
Phospho (Y)
Protein - Site of Modification
192
Peptide - Site of Modification
8
Peptide Start
185
Peptide End
196
CPTAC ID
CPTAC-929
Peptide Molecular Mass
1,431.6184
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 18.3 8.3 9.6 19.1 13.1 14.3 26.5 15.5 17.2 15 24 15
y9 (1+) 40.9 15.3 17.6 32.6 14.8 16.2 52.3 21.3 23.9 15 24 15
y10 (1+) 31.7 12.1 6 25.8 12.3 9.2 40.9 17.3 11 15 24 15
sum 28.7 8.6 7.4 21.6 10.7 7.3 35.9 13.7 10.4 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1775 Collapse assay details

Data source: Panorama

Official Gene Symbol
LCK
Peptide Modified Sequence
LIEDNEY[+80.0]TAR
Modification Type
Phospho (Y)
Protein - Site of Modification
394
Peptide - Site of Modification
7
Peptide Start
388
Peptide End
397
CPTAC ID
CPTAC-1775
Peptide Molecular Mass
1,302.5493
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.9 9.1 5.5 4.2 8 8.5 5.7 12.1 10.1 15 15 15
y4 (1+) 4.6 9.2 5 5.7 8.8 8 7.3 12.7 9.4 15 15 15
y5 (1+) 4.8 11.1 4.4 4.9 9.4 9.8 6.9 14.5 10.7 15 15 15
y6 (1+) 4.7 10.3 6.7 6.4 10.4 10.3 7.9 14.6 12.3 15 15 15
y7 (1+) 5.1 8.7 5.9 6 8.9 9.4 7.9 12.4 11.1 15 15 15
y8 (1+) 3.7 9.3 7 6.1 9.4 8.8 7.1 13.2 11.2 15 15 15
sum 4 9.3 5.6 5.6 9 8.7 6.9 12.9 10.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments