MED24, CPTAC-933 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MED24	ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100

Sequence Length (AA)	Molecular Weight (Da)
989	110305

Protein Name
Mediator of RNA polymerase II transcription subunit 24

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKVVNLKQAI	LQAWKERWSD	YQWAINMKKF	FPKGATWDIL	NLADALLEQA
60	70	80	90	100
MIGPSPNPLI	LSYLKYAISS	QMVSYSSVLT	AISKFDDFSR	DLCVQALLDI
110	120	130	140	150
MDMFCDRLSC	HGKAEECIGL	CRALLSALHW	LLRCTAASAE	RLREGLEAGT
160	170	180	190	200
PAAGEKQLAM	CLQRLEKTLS	STKNRALLHI	AKLEEASSWT	AIEHSLLKLG
210	220	230	240	250
EILANLSNPQ	LRSQAEQCGT	LIRSIPTMLS	VHAEQMHKTG	FPTVHAVILL
260	270	280	290	300
EGTMNLTGET	QSLVEQLTMV	KRMQHIPTPL	FVLEIWKACF	VGLIESPEGT
310	320	330	340	350
EELKWTAFTF	LKIPQVLVKL	KKYSHGDKDF	TEDVNCAFEF	LLKLTPLLDK
360	370	380	390	400
ADQRCNCDCT	NFLLQECGKQ	GLLSEASVNN	LMAKRKADRE	HAPQQKSGEN
410	420	430	440	450
ANIQPNIQLI	LRAEPTVTNI	LKTMDADHSK	SPEGLLGVLG	HMLSGKSLDL
460	470	480	490	500
LLAAAAATGK	LKSFARKFIN	LNEFTTYGSE	ESTKPASVRA	LLFDISFLML
510	520	530	540	550
CHVAQTYGSE	VILSESRTGA	EVPFFETWMQ	TCMPEEGKIL	NPDHPCFRPD
560	570	580	590	600
STKVESLVAL	LNNSSEMKLV	QMKWHEACLS	ISAAILEILN	AWENGVLAFE
610	620	630	640	650
SIQKITDNIK	GKVCSLAVCA	VAWLVAHVRM	LGLDEREKSL	QMIRQLAGPL
660	670	680	690	700
FSENTLQFYN	ERVVIMNSIL	ERMCADVLQQ	TATQIKFPST	GVDTMPYWNL
710	720	730	740	750
LPPKRPIKEV	LTDIFAKVLE	KGWVDSRSIH	IFDTLLHMGG	VYWFCNNLIK
760	770	780	790	800
ELLKETRKEH	TLRAVELLYS	IFCLDMQQVT	LVLLGHILPG	LLTDSSKWHS
810	820	830	840	850
LMDPPGTALA	KLAVWCALSS	YSSHKGQAST	RQKKRHREDI	EDYISLFPLD
860	870	880	890	900
DVQPSKLMRL	LSSNEDDANI	LSSPTDRSMS	SSLSASQLHT	VNMRDPLNRV
910	920	930	940	950
LANLFLLISS	ILGSRTAGPH	TQFVQWFMEE	CVDCLEQGGR	GSVLQFMPFT
960	970	980	989
TVSELVKVSA	MSSPKVVLAI	TDLSLPLGRQ	VAAKAIAAL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-933 Collapse assay details

Data source: Panorama

Official Gene Symbol: MED24
Peptide Modified Sequence: LLS[+80.0]SNEDDANILSSPTDR
Modification Type: Phospho (ST)
Protein - Site of Modification: 862
Peptide - Site of Modification: 3
Peptide Start: 860
Peptide End: 877

CPTAC ID: CPTAC-933
Peptide Molecular Mass: 2,025.8892
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	57.9	13.1	12.2	44	15.5	13.6	72.7	20.3	18.3	15	24	15
y6 (1+)	52.5	12	11.6	41.9	16.6	11.8	67.2	20.5	16.5	15	24	15
y7 (1+)	43.4	11.2	11.1	32.4	15.8	10.6	54.2	19.4	15.3	15	24	15
sum	48.9	11.3	10.8	34.8	15.7	10.7	60	19.3	15.2	15	24	15

MED24

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-933 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

MED24

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-933 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments