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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MED24 ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100
Sequence Length (AA) Molecular Weight (Da)
989 110305
Protein Name
Mediator of RNA polymerase II transcription subunit 24
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA
60 70 80 90 100
MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFSR DLCVQALLDI
110 120 130 140 150
MDMFCDRLSC HGKAEECIGL CRALLSALHW LLRCTAASAE RLREGLEAGT
160 170 180 190 200
PAAGEKQLAM CLQRLEKTLS STKNRALLHI AKLEEASSWT AIEHSLLKLG
210 220 230 240 250
EILANLSNPQ LRSQAEQCGT LIRSIPTMLS VHAEQMHKTG FPTVHAVILL
260 270 280 290 300
EGTMNLTGET QSLVEQLTMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT
310 320 330 340 350
EELKWTAFTF LKIPQVLVKL KKYSHGDKDF TEDVNCAFEF LLKLTPLLDK
360 370 380 390 400
ADQRCNCDCT NFLLQECGKQ GLLSEASVNN LMAKRKADRE HAPQQKSGEN
410 420 430 440 450
ANIQPNIQLI LRAEPTVTNI LKTMDADHSK SPEGLLGVLG HMLSGKSLDL
460 470 480 490 500
LLAAAAATGK LKSFARKFIN LNEFTTYGSE ESTKPASVRA LLFDISFLML
510 520 530 540 550
CHVAQTYGSE VILSESRTGA EVPFFETWMQ TCMPEEGKIL NPDHPCFRPD
560 570 580 590 600
STKVESLVAL LNNSSEMKLV QMKWHEACLS ISAAILEILN AWENGVLAFE
610 620 630 640 650
SIQKITDNIK GKVCSLAVCA VAWLVAHVRM LGLDEREKSL QMIRQLAGPL
660 670 680 690 700
FSENTLQFYN ERVVIMNSIL ERMCADVLQQ TATQIKFPST GVDTMPYWNL
710 720 730 740 750
LPPKRPIKEV LTDIFAKVLE KGWVDSRSIH IFDTLLHMGG VYWFCNNLIK
760 770 780 790 800
ELLKETRKEH TLRAVELLYS IFCLDMQQVT LVLLGHILPG LLTDSSKWHS
810 820 830 840 850
LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD
860 870 880 890 900
DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLHT VNMRDPLNRV
910 920 930 940 950
LANLFLLISS ILGSRTAGPH TQFVQWFMEE CVDCLEQGGR GSVLQFMPFT
960 970 980 989
TVSELVKVSA MSSPKVVLAI TDLSLPLGRQ VAAKAIAAL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-933 Collapse assay details

Data source: Panorama

Official Gene Symbol
MED24
Peptide Modified Sequence
LLS[+80.0]SNEDDANILSSPTDR
Modification Type
Phospho (ST)
Protein - Site of Modification
862
Peptide - Site of Modification
3
Peptide Start
860
Peptide End
877
CPTAC ID
CPTAC-933
Peptide Molecular Mass
2,025.8892
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 57.9 13.1 12.2 44 15.5 13.6 72.7 20.3 18.3 15 24 15
y6 (1+) 52.5 12 11.6 41.9 16.6 11.8 67.2 20.5 16.5 15 24 15
y7 (1+) 43.4 11.2 11.1 32.4 15.8 10.6 54.2 19.4 15.3 15 24 15
sum 48.9 11.3 10.8 34.8 15.7 10.7 60 19.3 15.2 15 24 15

Additional Resources and Comments