Overview Data source: UniProt

Official Gene Symbol Other Aliases
NBN NBS,NBS1,P95
Sequence Length (AA) Molecular Weight (Da)
754 84959
Protein Name
Nibrin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWKLLPAAGP AGGEPYRLLT GVEYVVGRKN CAILIENDQS ISRNHAVLTA
60 70 80 90 100
NFSVTNLSQT DEIPVLTLKD NSKYGTFVNE EKMQNGFSRT LKSGDGITFG
110 120 130 140 150
VFGSKFRIEY EPLVACSSCL DVSGKTALNQ AILQLGGFTV NNWTEECTHL
160 170 180 190 200
VMVSVKVTIK TICALICGRP IVKPEYFTEF LKAVESKKQP PQIESFYPPL
210 220 230 240 250
DEPSIGSKNV DLSGRQERKQ IFKGKTFIFL NAKQHKKLSS AVVFGGGEAR
260 270 280 290 300
LITEENEEEH NFFLAPGTCV VDTGITNSQT LIPDCQKKWI QSIMDMLQRQ
310 320 330 340 350
GLRPIPEAEI GLAVIFMTTK NYCDPQGHPS TGLKTTTPGP SLSQGVSVDE
360 370 380 390 400
KLMPSAPVNT TTYVADTESE QADTWDLSER PKEIKVSKME QKFRMLSQDA
410 420 430 440 450
PTVKESCKTS SNNNSMVSNT LAKMRIPNYQ LSPTKLPSIN KSKDRASQQQ
460 470 480 490 500
QTNSIRNYFQ PSTKKRERDE ENQEMSSCKS ARIETSCSLL EQTQPATPSL
510 520 530 540 550
WKNKEQHLSE NEPVDTNSDN NLFTDTDLKS IVKNSASKSH AAEKLRSNKK
560 570 580 590 600
REMDDVAIED EVLEQLFKDT KPELEIDVKV QKQEEDVNVR KRPRMDIETN
610 620 630 640 650
DTFSDEAVPE SSKISQENEI GKKRELKEDS LWSAKEISNN DKLQDDSEML
660 670 680 690 700
PKKLLLTEFR SLVIKNSTSR NPSGINDDYG QLKNFKKFKK VTYPGAGKLP
710 720 730 740 750
HIIGGSDLIA HHARKNTELE EWLRQEMEVQ NQHAKEESLA DDLFRYNPYL
754
KRRR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
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Assay Details for CPTAC-935 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Modified Sequence
IPNYQLS[+80.0]PTK
Modification Type
Phospho (ST)
Protein - Site of Modification
432
Peptide - Site of Modification
7
Peptide Start
426
Peptide End
435
CPTAC ID
CPTAC-935
Peptide Molecular Mass
1,239.5901
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7-98 (1+) 52.6 14.9 37.6 52.5 13.6 31.9 74.3 20.2 49.3 15 24 15
y8 (2+) 14.4 12 16.9 38.1 11.6 21.2 40.7 16.7 27.1 15 24 15
y9 (2+) 22.8 7.3 7.8 29.3 11 8.9 37.1 13.2 11.8 15 24 15
sum 20 7.2 8.1 27.9 10 10.4 34.3 12.3 13.2 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-3238 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Sequence
IPNYQLSPTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
426
Peptide End
435
CPTAC ID
CPTAC-3238
Peptide Molecular Mass
1,159.6237
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 19.9 8.5 7.4 20.3 8.3 7.6 28.4 11.9 10.6 15 15 15
y8 (1+) 11.9 9.6 4.7 23.3 15.8 10.7 26.2 18.5 11.7 15 15 15
y4 (1+) 28.5 10.5 5 35.1 14.8 10.2 45.2 18.1 11.4 15 15 15
sum 13.6 7.6 4 17.8 12.1 8.8 22.4 14.3 9.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3239 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Modified Sequence
IPNYQLS[+80.0]PTK
Modification Type
Phospho (ST)
Protein - Site of Modification
432
Peptide - Site of Modification
7
Peptide Start
426
Peptide End
435
CPTAC ID
CPTAC-3239
Peptide Molecular Mass
1,239.5901
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 19 24.5 13 38 27.3 20.9 42.5 36.7 24.6 15 15 15
y8 (1+) 18.2 13 9.8 42.5 18 12.1 46.2 22.2 15.6 15 15 15
y9-98 (2+) 17.6 11.1 8.2 47.2 14.5 12.7 50.4 18.3 15.1 15 15 15
y8-98 (1+) 32 13.3 11.1 47.2 13.7 20 57 19.1 22.9 15 15 15
sum 12.6 11.5 6.8 42.1 15.3 12.5 43.9 19.1 14.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-934 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Modified Sequence
TTTPGPSLS[+80.0]QGVSVDEK
Modification Type
Phospho (ST)
Protein - Site of Modification
343
Peptide - Site of Modification
9
Peptide Start
335
Peptide End
351
CPTAC ID
CPTAC-934
Peptide Molecular Mass
1,781.8084
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 28.6 8 10.1 23.1 11.3 14.8 36.8 13.8 17.9 15 24 15
y14 (2+) 33.1 8.7 9.6 24.2 10.4 12.2 41 13.6 15.5 15 24 15
y15 (2+) 48.5 11.4 8.5 40.3 11.6 14.1 63.1 16.3 16.5 15 24 15
sum 32.1 8.2 8.3 20.9 9.6 12.1 38.3 12.6 14.7 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-3236 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Sequence
TTTPGPSLSQGVSVDEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
335
Peptide End
351
CPTAC ID
CPTAC-3236
Peptide Molecular Mass
1,701.8421
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 86.1 26.9 20.6 90.6 28.8 20 125 39.4 28.7 15 15 15
y12 (2+) 72.5 49.6 54.3 64.7 53.6 76.5 97.2 73 93.8 15 15 15
y14 (2+) 25.5 14.5 17.3 25.5 14.2 16.7 36.1 20.3 24 15 15 15
sum 23.1 12.1 16.7 22.9 12.9 16.3 32.5 17.7 23.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3237 Collapse assay details

Data source: Panorama

Official Gene Symbol
NBN
Peptide Modified Sequence
TTTPGPSLS[+80.0]QGVSVDEK
Modification Type
Phospho (ST)
Protein - Site of Modification
343
Peptide - Site of Modification
9
Peptide Start
335
Peptide End
351
CPTAC ID
CPTAC-3237
Peptide Molecular Mass
1,781.8084
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 28.1 11.5 20 39.3 10.8 16.5 48.3 15.8 25.9 15 15 15
y14 (2+) 22 8.5 7.8 27.2 10.1 7 35 13.2 10.5 15 15 15
y15 (2+) 33.1 10.6 7.9 38.9 14.7 13.1 51.1 18.1 15.3 15 15 15
y5 (1+) 44.8 8.8 13.9 47 8.6 13.5 64.9 12.3 19.4 15 15 15
sum 12.5 6.6 7.1 24.3 8.3 5.9 27.3 10.6 9.2 15 15 15

Additional Resources and Comments