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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SGOL2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SGOL2 N/A
Sequence Length (AA) Molecular Weight (Da)
1265 144739
Protein Name
Shugoshin-like 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MECPVMETGS LFTSGIKRHL KDKRISKTTK LNVSLASKIK TKILNNSSIF
60 70 80 90 100
KISLKHNNRA LAQALSREKE NSRRITTEKM LLQKEVEKLN FENTFLRLKL
110 120 130 140 150
NNLNKKLIDI EALMNNNLIT AIEMSSLSEF HQSSFLLSAS KKKRISKQCK
160 170 180 190 200
LMRLPFARVP LTSNDDEDED KEKMQCDNNI KSKTLPDIPS SGSTTQPLST
210 220 230 240 250
QDNSEVLFLK ENNQNVYGLD DSEHISSIVD VPPRESHSHS DQSSKTSLMS
260 270 280 290 300
EMRNAQSIGR RWEKPSPSNV TERKKRGSSW ESNNLSADTP CATVLDKQHI
310 320 330 340 350
SSPELNCNNE INGHTNETNT EMQRNKQDLP GLSSESAREP NAECMNQIED
360 370 380 390 400
NDDFQLQKTV YDADMDLTAS EVSKIVTVST GIKKKSNKKT NEHGMKTFRK
410 420 430 440 450
VKDSSSEKKR ERSKRQFKNS SDVDIGEKIE NRTERSDVLD GKRGAEDPGF
460 470 480 490 500
IFNNEQLAQM NEQLAQVNEL KKMTLQTGFE QGDRENVLCN KKEKRITNEQ
510 520 530 540 550
EETYSLSQSS GKFHQESKFD KGQNSLTCNK SKASRQTFVI HKLEKDNLLP
560 570 580 590 600
NQKDKVTIYE NLDVTNEFHT ANLSTKDNGN LCDYGTHNIL DLKKYVTDIQ
610 620 630 640 650
PSEQNESNIN KLRKKVNRKT EIISGMNHMY EDNDKDVVHG LKKGNFFFKT
660 670 680 690 700
QEDKEPISEN IEVSKELQIP ALSTRDNENQ CDYRTQNVLG LQKQITNMYP
710 720 730 740 750
VQQNESKVNK KLRQKVNRKT EIISEVNHLD NDKSIEYTVK SHSLFLTQKD
760 770 780 790 800
KEIIPGNLED PSEFETPALS TKDSGNLYDS EIQNVLGVKH GHDMQPACQN
810 820 830 840 850
DSKIGKKPRL NVCQKSEIIP ETNQIYENDN KGVHDLEKDN FFSLTPKDKE
860 870 880 890 900
TISENLQVTN EFQTVDLLIK DNGNLCDYDT QNILELKKYV TDRKSAEQNE
910 920 930 940 950
SKINKLRNKV NWKTEIISEM NQIYEDNDKD AHVQESYTKD LDFKVNKSKQ
960 970 980 990 1000
KLECQDIINK HYMEVNSNEK ESCDQILDSY KVVKKRKKES SCKAKNILTK
1010 1020 1030 1040 1050
AKNKLASQLT ESSQTSISLE SDLKHITSEA DSDPGNPVEL CKTQKQSTTT
1060 1070 1080 1090 1100
LNKKDLPFVE EIKEGECQVK KVNKMTSKSK KRKTSIDPSP ESHEVMERIL
1110 1120 1130 1140 1150
DSVQGKSTVS EQADKENNLE NEKMVKNKPD FYTKAFRSLS EIHSPNIQDS
1160 1170 1180 1190 1200
SFDSVREGLV PLSVSSGKNV IIKENFALEC SPAFQVSDDE HEKMNKMKFK
1210 1220 1230 1240 1250
VNRRTQKSGI GDRPLQDLSN TSFVSNNTAE SENKSEDLSS ERTSRRRRCT
1260 1265
PFYFKEPSLR DKMRR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-946 Collapse assay details

Data source: Panorama

Official Gene Symbol
SGOL2
Peptide Modified Sequence
DNFFSLT[+80.0]PK
Modification Type
Phospho (ST)
Protein - Site of Modification
845
Peptide - Site of Modification
7
Peptide Start
839
Peptide End
847
CPTAC ID
CPTAC-946
Peptide Molecular Mass
1,147.4951
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 27.2 11.3 8.4 28.1 14.8 12.3 39.1 18.6 14.9 15 24 15
y6 (1+) 29.1 9.7 10.7 19 13 9.6 34.8 16.2 14.4 15 24 15
y7 (1+) 33.8 16.3 27.1 23.8 17.7 25.3 41.3 24.1 37.1 15 24 15
sum 28.4 8.4 6.5 22.2 10.4 8.8 36 13.4 10.9 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments