SNRPC, CPTAC-947 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SNRPC	N/A

Sequence Length (AA)	Molecular Weight (Da)
159	17394

Protein Name
U1 small nuclear ribonucleoprotein C

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPKFYCDYCD	TYLTHDSPSV	RKTHCSGRKH	KENVKDYYQK	WMEEQAQSLI
60	70	80	90	100
DKTTAAFQQG	KIPPTPFSAP	PPAGAMIPPP	PSLPGPPRPG	MMPAPHMGGP
110	120	130	140	150
PMMPMMGPPP	PGMMPVGPAP	GMRPPMGGHM	PMMPGPPMMR	PPARPMMVPT
159
RPGMTRPDR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-947 Collapse assay details

Data source: Panorama

Official Gene Symbol: SNRPC
Peptide Modified Sequence: FYC[+57.0]DYC[+57.0]DTYLTHDS[+80.0]PSVR
Modification Type: Carbamidomethyl Cysteine, Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification: 6, 9, 17
Peptide - Site of Modification: 3, 6, 14
Peptide Start: 4
Peptide End: 21

CPTAC ID: CPTAC-947
Peptide Molecular Mass: 2,377.9021
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y15 (2+)	54.7	36	23.4	59	34	18.6	80.5	49.5	29.9	10	21	15
y17 (2+)	26.4	60.6	93.5	37.8	53.5	121.3	46.1	80.8	153.2	10	21	15
y4 (1+)	24.5	17.4	10.4	58.4	28.5	20.4	63.3	33.4	22.9	10	21	15
y8 (1+)	71.1	24	54.2	61	31.4	87.6	93.7	39.5	103	10	21	15
sum	20.8	17	11.9	35.5	24.1	21.4	41.1	29.5	24.5	10	21	15

SNRPC

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-947 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

SNRPC

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-947 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments