C16orf54, CPTAC-959 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C16orf54	N/A

Sequence Length (AA)	Molecular Weight (Da)
224	24360

Protein Name
Transmembrane protein C16orf54

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPLTPEPPSG	RVEGPPAWEA	APWPSLPCGP	CIPIMLVLAT	LAALFILTTA
60	70	80	90	100
VLAERLFRRA	LRPDPSHRAP	TLVWRPGGEL	WIEPMGTARE	RSEDWYGSAV
110	120	130	140	150
PLLTDRAPEP	PTQVGTLEAR	ATAPPAPSAP	NSAPSNLGPQ	TVLEVPARST
160	170	180	190	200
FWGPQPWEGR	PPATGLVSWA	EPEQRPEASV	QFGSPQARRQ	RPGSPDPEWG
210	220	224
LQPRVTLEQI	SAFWKREGRT	SVGF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-959 Collapse assay details

Data source: Panorama

Official Gene Symbol: C16orf54
Peptide Modified Sequence: SEDWYGS[+80.0]AVPLLTDR
Modification Type: Phospho (ST)
Protein - Site of Modification: 98
Peptide - Site of Modification: 7
Peptide Start: 92
Peptide End: 106

CPTAC ID: CPTAC-959
Peptide Molecular Mass: 1,787.7767
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b9-98 (1+)	42.9	26.3	24.9	34.6	43.4	49.1	55.1	50.7	55.1	9	19	11
b8-98 (1+)	35.9	48.7	32.4	46.1	52.6	42.5	58.4	71.7	53.4	9	19	11
y6 (1+)	51.3	10.1	11.3	41	26.5	9.8	65.7	28.4	15	9	19	11
sum	46.5	11.2	15.3	39.5	27.3	21.8	61	29.5	26.6	9	19	11

C16orf54

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-959 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

C16orf54

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-959 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments