TMEM230, CPTAC-960 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TMEM230	C20orf30

Sequence Length (AA)	Molecular Weight (Da)
120	13188

Protein Name
Transmembrane protein 230

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MMPSRTNLAT	GIPSSKVKYS	RLSSTDDGYI	DLQFKKTPPK	IPYKAIALAT
60	70	80	90	100
VLFLIGAFLI	IIGSLLLSGY	ISKGGADRAV	PVLIIGILVF	LPGFYHLRIA
110	120
YYASKGYRGY	SYDDIPDFDD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-960 Collapse assay details

Data source: Panorama

Official Gene Symbol: TMEM230
Peptide Modified Sequence: LSS[+80.0]TDDGYIDLQFK
Modification Type: Phospho (ST)
Protein - Site of Modification: 24
Peptide - Site of Modification: 3
Peptide Start: 22
Peptide End: 35

CPTAC ID: CPTAC-960
Peptide Molecular Mass: 1,680.7284
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

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Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	60.2	18	8.8	66.4	34.9	11.7	89.6	39.3	14.6	15	24	15
y6 (1+)	46.2	19.4	7.4	131.9	49.2	16.6	139.8	52.9	18.2	15	24	15
y8 (1+)	36.7	13.8	8.1	48	18.9	14.2	60.4	23.4	16.3	15	24	15
sum	37.2	12.8	6.6	88.1	35	12.1	95.6	37.3	13.8	15	24	15

TMEM230

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-960 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TMEM230

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-960 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments