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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDC42EP3 BORG2,CEP3
Sequence Length (AA) Molecular Weight (Da)
254 27678
Protein Name
Cdc42 effector protein 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPAKTPIYLK AANNKKGKKF KLRDILSPDM ISPPLGDFRH TIHIGKEGQH
60 70 80 90 100
DVFGDISFLQ GNYELLPGNQ EKAHLGQFPG HNEFFRANST SDSVFTETPS
110 120 130 140 150
PVLKNAISLP TIGGSQALML PLLSPVTFNS KQESFGPAKL PRLSCEPVME
160 170 180 190 200
EKAQEKSSLL ENGTVHQGDT SWGSSGSASQ SSQGRDSHSS SLSEQYPDWP
210 220 230 240 250
AEDMFDHPTP CELIKGKTKS EESLSDLTGS LLSLQLDLGP SLLDEVLNVM
254
DKNK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-961 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC42EP3
Peptide Modified Sequence
ANS[+80.0]TSDSVFTETPSPVLK
Modification Type
Phospho (ST)
Protein - Site of Modification
89
Peptide - Site of Modification
3
Peptide Start
87
Peptide End
104
CPTAC ID
CPTAC-961
Peptide Molecular Mass
1,958.8874
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 41.6 10.2 15.1 24.3 12.6 14.9 48.2 16.2 21.2 15 24 15
y7 (1+) 31 14.5 7.4 23.7 12.1 16.7 39 18.9 18.3 15 24 15
y10 (1+) 33.8 12.3 10.4 23.5 12.1 11.1 41.2 17.3 15.2 15 24 15
sum 33.8 10.7 9.1 21.5 11.1 10.4 40.1 15.4 13.8 15 24 15

Additional Resources and Comments