CDC42EP3, CPTAC-961 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDC42EP3	BORG2, CEP3

Sequence Length (AA)	Molecular Weight (Da)
254	27678

Protein Name
Cdc42 effector protein 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPAKTPIYLK	AANNKKGKKF	KLRDILSPDM	ISPPLGDFRH	TIHIGKEGQH
60	70	80	90	100
DVFGDISFLQ	GNYELLPGNQ	EKAHLGQFPG	HNEFFRANST	SDSVFTETPS
110	120	130	140	150
PVLKNAISLP	TIGGSQALML	PLLSPVTFNS	KQESFGPAKL	PRLSCEPVME
160	170	180	190	200
EKAQEKSSLL	ENGTVHQGDT	SWGSSGSASQ	SSQGRDSHSS	SLSEQYPDWP
210	220	230	240	250
AEDMFDHPTP	CELIKGKTKS	EESLSDLTGS	LLSLQLDLGP	SLLDEVLNVM
254
DKNK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-961 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDC42EP3
Peptide Modified Sequence: ANS[+80.0]TSDSVFTETPSPVLK
Modification Type: Phospho (ST)
Protein - Site of Modification: 89
Peptide - Site of Modification: 3
Peptide Start: 87
Peptide End: 104

CPTAC ID: CPTAC-961
Peptide Molecular Mass: 1,958.8874
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

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Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	41.6	10.2	15.1	24.3	12.6	14.9	48.2	16.2	21.2	15	24	15
y7 (1+)	31	14.5	7.4	23.7	12.1	16.7	39	18.9	18.3	15	24	15
y10 (1+)	33.8	12.3	10.4	23.5	12.1	11.1	41.2	17.3	15.2	15	24	15
sum	33.8	10.7	9.1	21.5	11.1	10.4	40.1	15.4	13.8	15	24	15

CDC42EP3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-961 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CDC42EP3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-961 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments