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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

DOCK2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DOCK2 KIAA0209
Sequence Length (AA) Molecular Weight (Da)
1830 211948
Protein Name
Dedicator of cytokinesis protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPWRKADKE RHGVAIYNFQ GSGAPQLSLQ IGDVVRIQET CGDWYRGYLI
60 70 80 90 100
KHKMLQGIFP KSFIHIKEVT VEKRRNTENI IPAEIPLAQE VTTTLWEWGS
110 120 130 140 150
IWKQLYVASK KERFLQVQSM MYDLMEWRSQ LLSGTLPKDE LKELKQKVTS
160 170 180 190 200
KIDYGNKILE LDLIVRDEDG NILDPDNTSV ISLFHAHEEA TDKITERIKE
210 220 230 240 250
EMSKDQPDYA MYSRISSSPT HSLYVFVRNF VCRIGEDAEL FMSLYDPNKQ
260 270 280 290 300
TVISENYLVR WGSRGFPKEI EMLNNLKVVF TDLGNKDLNR DKIYLICQIV
310 320 330 340 350
RVGKMDLKDT GAKKCTQGLR RPFGVAVMDI TDIIKGKAES DEEKQHFIPF
360 370 380 390 400
HPVTAENDFL HSLLGKVIAS KGDSGGQGLW VTMKMLVGDI IQIRKDYPHL
410 420 430 440 450
VDRTTVVARK LGFPEIIMPG DVRNDIYITL LQGDFDKYNK TTQRNVEVIM
460 470 480 490 500
CVCAEDGKTL PNAICVGAGD KPMNEYRSVV YYQVKQPRWM ETVKVAVPIE
510 520 530 540 550
DMQRIHLRFM FRHRSSLESK DKGEKNFAMS YVKLMKEDGT TLHDGFHDLV
560 570 580 590 600
VLKGDSKKME DASAYLTLPS YRHHVENKGA TLSRSSSSVG GLSVSSRDVF
610 620 630 640 650
SISTLVCSTK LTQNVGLLGL LKWRMKPQLL QENLEKLKIV DGEEVVKFLQ
660 670 680 690 700
DTLDALFNIM MEHSQSDEYD ILVFDALIYI IGLIADRKFQ HFNTVLEAYI
710 720 730 740 750
QQHFSATLAY KKLMTVLKTY LDTSSRGEQC EPILRTLKAL EYVFKFIVRS
760 770 780 790 800
RTLFSQLYEG KEQMEFEESM RRLFESINNL MKSQYKTTIL LQVAALKYIP
810 820 830 840 850
SVLHDVEMVF DAKLLSQLLY EFYTCIPPVK LQKQKVQSMN EIVQSNLFKK
860 870 880 890 900
QECRDILLPV ITKELKELLE QKDDMQHQVL ERKYCVELLN SILEVLSYQD
910 920 930 940 950
AAFTYHHIQE IMVQLLRTVN RTVITMGRDH ILISHFVACM TAILNQMGDQ
960 970 980 990 1000
HYSFYIETFQ TSSELVDFLM ETFIMFKDLI GKNVYPGDWM AMSMVQNRVF
1010 1020 1030 1040 1050
LRAINKFAET MNQKFLEHTN FEFQLWNNYF HLAVAFITQD SLQLEQFSHA
1060 1070 1080 1090 1100
KYNKILNKYG DMRRLIGFSI RDMWYKLGQN KICFIPGMVG PILEMTLIPE
1110 1120 1130 1140 1150
AELRKATIPI FFDMMLCEYQ RSGDFKKFEN EIILKLDHEV EGGRGDEQYM
1160 1170 1180 1190 1200
QLLESILMEC AAEHPTIAKS VENFVNLVKG LLEKLLDYRG VMTDESKDNR
1210 1220 1230 1240 1250
MSCTVNLLNF YKDNNREEMY IRYLYKLRDL HLDCDNYTEA AYTLLLHTWL
1260 1270 1280 1290 1300
LKWSDEQCAS QVMQTGQQHP QTHRQLKETL YETIIGYFDK GKMWEEAISL
1310 1320 1330 1340 1350
CKELAEQYEM EIFDYELLSQ NLIQQAKFYE SIMKILRPKP DYFAVGYYGQ
1360 1370 1380 1390 1400
GFPSFLRNKV FIYRGKEYER REDFQMQLMT QFPNAEKMNT TSAPGDDVKN
1410 1420 1430 1440 1450
APGQYIQCFT VQPVLDEHPR FKNKPVPDQI INFYKSNYVQ RFHYSRPVRR
1460 1470 1480 1490 1500
GTVDPENEFA SMWIERTSFV TAYKLPGILR WFEVVHMSQT TISPLENAIE
1510 1520 1530 1540 1550
TMSTANEKIL MMINQYQSDE TLPINPLSML LNGIVDPAVM GGFAKYEKAF
1560 1570 1580 1590 1600
FTEEYVRDHP EDQDKLTHLK DLIAWQIPFL GAGIKIHEKR VSDNLRPFHD
1610 1620 1630 1640 1650
RMEECFKNLK MKVEKEYGVR EMPDFDDRRV GRPRSMLRSY RQMSIISLAS
1660 1670 1680 1690 1700
MNSDCSTPSK PTSESFDLEL ASPKTPRVEQ EEPISPGSTL PEVKLRRSKK
1710 1720 1730 1740 1750
RTKRSSVVFA DEKAAAESDL KRLSRKHEFM SDTNLSEHAA IPLKASVLSQ
1760 1770 1780 1790 1800
MSFASQSMPT IPALALSVAG IPGLDEANTS PRLSQTFLQL SDGDKKTLTR
1810 1820 1830
KKVNQFFKTM LASKSAEEGK QIPDSLSTDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-964 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK2
Peptide Modified Sequence
VEQEEPIS[+80.0]PGSTLPEVK
Modification Type
Phospho (ST)
Protein - Site of Modification
1685
Peptide - Site of Modification
8
Peptide Start
1678
Peptide End
1694
CPTAC ID
CPTAC-964
Peptide Molecular Mass
1,917.8973
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 32.3 10.7 13.1 21.9 12.5 14.6 39 16.5 19.6 14 24 15
y9 (1+) 30.3 19.7 37.2 59.6 21.1 52.2 66.9 28.9 64.1 14 24 15
b7 (1+) 37.9 24.9 18.6 51.6 26.7 23 64 36.5 29.6 14 24 15
sum 21.4 10.4 20.1 20 12.2 29.1 29.3 16 35.4 14 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1604 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK2
Peptide Sequence
DILLPVITK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
855
Peptide End
863
CPTAC ID
CPTAC-1604
Peptide Molecular Mass
1,010.6376
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 16.7 4.3 3.5 26.7 7.9 5.6 31.5 9 6.6 15 15 15
y6 (1+) 24.8 5.7 1.6 34.1 8.2 6.3 42.2 10 6.5 15 15 15
y7 (1+) 12.7 1.5 1.2 13.1 4.5 4.2 18.2 4.7 4.4 15 15 15
sum 8.6 2.4 1.3 15.5 5.6 3.7 17.7 6.1 3.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments