Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KIF4A KIF4
Sequence Length (AA) Molecular Weight (Da)
1232 139881
Protein Name
Chromosome-associated kinesin KIF4A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKEEVKGIPV RVALRCRPLV PKEISEGCQM CLSFVPGEPQ VVVGTDKSFT
60 70 80 90 100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG
110 120 130 140 150
AYTAEQENEP TVGVIPRVIQ LLFKEIDKKS DFEFTLKVSY LEIYNEEILD
160 170 180 190 200
LLCPSREKAQ INIREDPKEG IKIVGLTEKT VLVALDTVSC LEQGNNSRTV
210 220 230 240 250
ASTAMNSQSS RSHAIFTISL EQRKKSDKNS SFRSKLHLVD LAGSERQKKT
260 270 280 290 300
KAEGDRLKEG ININRGLLCL GNVISALGDD KKGGFVPYRD SKLTRLLQDS
310 320 330 340 350
LGGNSHTLMI ACVSPADSNL EETLNTLRYA DRARKIKNKP IVNIDPQTAE
360 370 380 390 400
LNHLKQQVQQ LQVLLLQAHG GTLPGSITVE PSENLQSLME KNQSLVEENE
410 420 430 440 450
KLSRGLSEAA GQTAQMLERI ILTEQANEKM NAKLEELRQH AACKLDLQKL
460 470 480 490 500
VETLEDQELK ENVEIICNLQ QLITQLSDET VACMAAAIDT AVEQEAQVET
510 520 530 540 550
SPETSRSSDA FTTQHALRQA QMSKELVELN KALALKEALA RKMTQNDSQL
560 570 580 590 600
QPIQYQYQDN IKELELEVIN LQKEKEELVL ELQTAKKDAN QAKLSERRRK
610 620 630 640 650
RLQELEGQIA DLKKKLNEQS KLLKLKESTE RTVSKLNQEI RMMKNQRVQL
660 670 680 690 700
MRQMKEDAEK FRQWKQKKDK EVIQLKERDR KRQYELLKLE RNFQKQSNVL
710 720 730 740 750
RRKTEEAAAA NKRLKDALQK QREVADKRKE TQSRGMEGTA ARVKNWLGNE
760 770 780 790 800
IEVMVSTEEA KRHLNDLLED RKILAQDVAQ LKEKKESGEN PPPKLRRRTF
810 820 830 840 850
SLTEVRGQVS ESEDSITKQI ESLETEMEFR SAQIADLQQK LLDAESEDRP
860 870 880 890 900
KQRWENIATI LEAKCALKYL IGELVSSKIQ VSKLESSLKQ SKTSCADMQK
910 920 930 940 950
MLFEERNHFA EIETELQAEL VRMEQQHQEK VLYLLSQLQQ SQMAEKQLEE
960 970 980 990 1000
SVSEKEQQLL STLKCQDEEL EKMREVCEQN QQLLRENEII KQKLTLLQVA
1010 1020 1030 1040 1050
SRQKHLPKDT LLSPDSSFEY VPPKPKPSRV KEKFLEQSMD IEDLKYCSEH
1060 1070 1080 1090 1100
SVNEHEDGDG DDDEGDDEEW KPTKLVKVSR KNIQGCSCKG WCGNKQCGCR
1110 1120 1130 1140 1150
KQKSDCGVDC CCDPTKCRNR QQGKDSLGTV ERTQDSEGSF KLEDPTEVTP
1160 1170 1180 1190 1200
GLSFFNPVCA TPNSKILKEM CDVEQVLSKK TPPAPSPFDL PELKHVATEY
1210 1220 1230 1232
QENKAPGKKK KRALASNTSF FSGCSPIEEE AH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-971 Collapse assay details

Data source: Panorama

Official Gene Symbol
KIF4A
Peptide Modified Sequence
TFS[+80.0]LTEVR
Modification Type
Phospho (ST)
Protein - Site of Modification
801
Peptide - Site of Modification
3
Peptide Start
799
Peptide End
806
CPTAC ID
CPTAC-971
Peptide Molecular Mass
1,031.4689
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.9 8 15.5 19.5 5.8 15.7 31.6 9.9 22.1 15 24 15
y6-98 (1+) 25.8 5.4 4.6 20.5 5.9 7 33 8 8.4 15 24 15
y6 (1+) 24.9 4.9 5.2 23.2 5 6.3 34 7 8.2 15 24 15
sum 24.8 4.3 4.2 20.7 4.2 6.7 32.3 6 7.9 15 24 15

Additional Resources and Comments