KIF4A, CPTAC-971 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KIF4A	KIF4

Sequence Length (AA)	Molecular Weight (Da)
1232	139881

Protein Name
Chromosome-associated kinesin KIF4A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKEEVKGIPV	RVALRCRPLV	PKEISEGCQM	CLSFVPGEPQ	VVVGTDKSFT
60	70	80	90	100
YDFVFDPSTE	QEEVFNTAVA	PLIKGVFKGY	NATVLAYGQT	GSGKTYSMGG
110	120	130	140	150
AYTAEQENEP	TVGVIPRVIQ	LLFKEIDKKS	DFEFTLKVSY	LEIYNEEILD
160	170	180	190	200
LLCPSREKAQ	INIREDPKEG	IKIVGLTEKT	VLVALDTVSC	LEQGNNSRTV
210	220	230	240	250
ASTAMNSQSS	RSHAIFTISL	EQRKKSDKNS	SFRSKLHLVD	LAGSERQKKT
260	270	280	290	300
KAEGDRLKEG	ININRGLLCL	GNVISALGDD	KKGGFVPYRD	SKLTRLLQDS
310	320	330	340	350
LGGNSHTLMI	ACVSPADSNL	EETLNTLRYA	DRARKIKNKP	IVNIDPQTAE
360	370	380	390	400
LNHLKQQVQQ	LQVLLLQAHG	GTLPGSITVE	PSENLQSLME	KNQSLVEENE
410	420	430	440	450
KLSRGLSEAA	GQTAQMLERI	ILTEQANEKM	NAKLEELRQH	AACKLDLQKL
460	470	480	490	500
VETLEDQELK	ENVEIICNLQ	QLITQLSDET	VACMAAAIDT	AVEQEAQVET
510	520	530	540	550
SPETSRSSDA	FTTQHALRQA	QMSKELVELN	KALALKEALA	RKMTQNDSQL
560	570	580	590	600
QPIQYQYQDN	IKELELEVIN	LQKEKEELVL	ELQTAKKDAN	QAKLSERRRK
610	620	630	640	650
RLQELEGQIA	DLKKKLNEQS	KLLKLKESTE	RTVSKLNQEI	RMMKNQRVQL
660	670	680	690	700
MRQMKEDAEK	FRQWKQKKDK	EVIQLKERDR	KRQYELLKLE	RNFQKQSNVL
710	720	730	740	750
RRKTEEAAAA	NKRLKDALQK	QREVADKRKE	TQSRGMEGTA	ARVKNWLGNE
760	770	780	790	800
IEVMVSTEEA	KRHLNDLLED	RKILAQDVAQ	LKEKKESGEN	PPPKLRRRTF
810	820	830	840	850
SLTEVRGQVS	ESEDSITKQI	ESLETEMEFR	SAQIADLQQK	LLDAESEDRP
860	870	880	890	900
KQRWENIATI	LEAKCALKYL	IGELVSSKIQ	VSKLESSLKQ	SKTSCADMQK
910	920	930	940	950
MLFEERNHFA	EIETELQAEL	VRMEQQHQEK	VLYLLSQLQQ	SQMAEKQLEE
960	970	980	990	1000
SVSEKEQQLL	STLKCQDEEL	EKMREVCEQN	QQLLRENEII	KQKLTLLQVA
1010	1020	1030	1040	1050
SRQKHLPKDT	LLSPDSSFEY	VPPKPKPSRV	KEKFLEQSMD	IEDLKYCSEH
1060	1070	1080	1090	1100
SVNEHEDGDG	DDDEGDDEEW	KPTKLVKVSR	KNIQGCSCKG	WCGNKQCGCR
1110	1120	1130	1140	1150
KQKSDCGVDC	CCDPTKCRNR	QQGKDSLGTV	ERTQDSEGSF	KLEDPTEVTP
1160	1170	1180	1190	1200
GLSFFNPVCA	TPNSKILKEM	CDVEQVLSKK	TPPAPSPFDL	PELKHVATEY
1210	1220	1230	1232
QENKAPGKKK	KRALASNTSF	FSGCSPIEEE	AH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-971 Collapse assay details

Data source: Panorama

Official Gene Symbol: KIF4A
Peptide Modified Sequence: TFS[+80.0]LTEVR
Modification Type: Phospho (ST)
Protein - Site of Modification: 801
Peptide - Site of Modification: 3
Peptide Start: 799
Peptide End: 806

CPTAC ID: CPTAC-971
Peptide Molecular Mass: 1,031.4689
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	24.9	8	15.5	19.5	5.8	15.7	31.6	9.9	22.1	15	24	15
y6-98 (1+)	25.8	5.4	4.6	20.5	5.9	7	33	8	8.4	15	24	15
y6 (1+)	24.9	4.9	5.2	23.2	5	6.3	34	7	8.2	15	24	15
sum	24.8	4.3	4.2	20.7	4.2	6.7	32.3	6	7.9	15	24	15

KIF4A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-971 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

KIF4A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-971 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments