Overview Data source: UniProt

Official Gene Symbol Other Aliases
LAT2 LAB,NTAL,WBS15,WBSCR15,WBSCR5
Sequence Length (AA) Molecular Weight (Da)
243 26550
Protein Name
Linker for activation of T-cells family member 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSGTELLWP GAALLVLLGV AASLCVRCSR PGAKRSEKIY QQRSLREDQQ
60 70 80 90 100
SFTGSRTYSL VGQAWPGPLA DMAPTRKDKL LQFYPSLEDP ASSRYQNFSK
110 120 130 140 150
GSRHGSEEAY IDPIAMEYYN WGRFSKPPED DDANSYENVL ICKQKTTETG
160 170 180 190 200
AQQEGIGGLC RGDLSLSLAL KTGPTSGLCP SASPEEDEES EDYQNSASIH
210 220 230 240 243
QWRESRKVMG QLQREASPGP VGSPDEEDGE PDYVNGEVAA TEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-972 Collapse assay details

Data source: Panorama

Official Gene Symbol
LAT2
Peptide Modified Sequence
LLQFYPS[+80.0]LEDPASSR
Modification Type
Phospho (ST)
Protein - Site of Modification
86
Peptide - Site of Modification
7
Peptide Start
80
Peptide End
94
CPTAC ID
CPTAC-972
Peptide Molecular Mass
1,801.8288
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 33.6 19.1 15 35 21.9 NA 48.5 29.1 NA 8 11 3
y10-98 (1+) 39.2 11.8 11.4 34.9 23.4 NA 52.5 26.2 NA 8 11 3
y10 (1+) 54.8 9.8 19.2 51.6 25.3 NA 75.3 27.1 NA 8 11 3
sum 39.7 9.3 11.6 35.1 23.3 NA 53 25.1 NA 8 11 3

Additional Resources and Comments