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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAVS

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAVS IPS1, KIAA1271, VISA
Sequence Length (AA) Molecular Weight (Da)
540 56528
Protein Name
Mitochondrial antiviral-signaling protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPFAEDKTYK YICRNFSNFC NVDVVEILPY LPCLTARDQD RLRATCTLSG
60 70 80 90 100
NRDTLWHLFN TLQRRPGWVE YFIAALRGCE LVDLADEVAS VYQSYQPRTS
110 120 130 140 150
DRPPDPLEPP SLPAERPGPP TPAAAHSIPY NSCREKEPSY PMPVQETQAP
160 170 180 190 200
ESPGENSEQA LQTLSPRAIP RNPDGGPLES SSDLAALSPL TSSGHQEQDT
210 220 230 240 250
ELGSTHTAGA TSSLTPSRGP VSPSVSFQPL ARSTPRASRL PGPTGSVVST
260 270 280 290 300
GTSFSSSSPG LASAGAAEGK QGAESDQAEP IICSSGAEAP ANSLPSKVPT
310 320 330 340 350
TLMPVNTVAL KVPANPASVS TVPSKLPTSS KPPGAVPSNA LTNPAPSKLP
360 370 380 390 400
INSTRAGMVP SKVPTSMVLT KVSASTVPTD GSSRNEETPA APTPAGATGG
410 420 430 440 450
SSAWLDSSSE NRGLGSELSK PGVLASQVDS PFSGCFEDLA ISASTSLGMG
460 470 480 490 500
PCHGPEENEY KSEGTFGIHV AENPSIQLLE GNPGPPADPD GGPRPQADRK
510 520 530 540
FQEREVPCHR PSPGALWLQV AVTGVLVVTL LVVLYRRRLH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-977 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAVS
Peptide Modified Sequence
GPVS[+80.0]PSVSFQPLAR
Modification Type
Phospho (ST)
Protein - Site of Modification
222
Peptide - Site of Modification
4
Peptide Start
219
Peptide End
232
CPTAC ID
CPTAC-977
Peptide Molecular Mass
1,520.7388
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 36.4 12.8 8.3 24 14.2 7.1 43.6 19.1 10.9 15 24 15
y7 (1+) 40.4 9.8 7.8 21.9 12.9 9.4 46 16.2 12.2 15 24 15
y10 (1+) 41.5 10.8 11.1 22.8 13.3 11.5 47.4 17.1 16 15 24 15
sum 38.6 10.2 6.7 21.7 13 7 44.3 16.5 9.7 15 24 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments