Overview Data source: UniProt

Official Gene Symbol Other Aliases
MARCKS MACS,PRKCSL
Sequence Length (AA) Molecular Weight (Da)
332 31555
Protein Name
Myristoylated alanine-rich C-kinase substrate
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAQFSKTAA KGEAAAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA
60 70 80 90 100
ESGAKEELQA NGSAPAADKE EPAAAGSGAA SPSAAEKGEP AAAAAPEAGA
110 120 130 140 150
SPVEKEAPAE GEAAEPGSPT AAEGEAASAA SSTSSPKAED GATPSPSNET
160 170 180 190 200
PKKKKKRFSF KKSFKLSGFS FKKNKKEAGE GGEAEAPAAE GGKDEAAGGA
210 220 230 240 250
AAAAAEAGAA SGEQAAAPGE EAAAGEEGAA GGDPQEAKPQ EAAVAPEKPP
260 270 280 290 300
ASDETKAAEE PSKVEEKKAE EAGASAAACE APSAAGPGAP PEQEAAPAEE
310 320 330 332
PAAAAASSAC AAPSQEAQPE CSPEAPPAEA AE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-976 Collapse assay details

Data source: Panorama

Official Gene Symbol
MARCKS
Peptide Modified Sequence
GEPAAAAAPEAGAS[+80.0]PVEK
Modification Type
Phospho (ST)
Protein - Site of Modification
101
Peptide - Site of Modification
14
Peptide Start
88
Peptide End
105
CPTAC ID
CPTAC-976
Peptide Molecular Mass
1,701.7611
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16 (2+) 28 10.7 11.3 17.3 10.6 12.6 32.9 15.1 16.9 15 24 15
y10 (1+) 22.7 11.8 11.5 22.9 13.6 15.5 32.2 18 19.3 15 24 15
y11 (1+) 29.8 9.7 14.9 39.8 13.5 18 49.7 16.6 23.4 15 24 15
sum 22.9 10.5 11.2 22.3 11.9 14.2 32 15.9 18.1 15 24 15

Additional Resources and Comments