MARCKS, CPTAC-976 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MARCKS	MACS, PRKCSL

Sequence Length (AA)	Molecular Weight (Da)
332	31555

Protein Name
Myristoylated alanine-rich C-kinase substrate

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAQFSKTAA	KGEAAAERPG	EAAVASSPSK	ANGQENGHVK	VNGDASPAAA
60	70	80	90	100
ESGAKEELQA	NGSAPAADKE	EPAAAGSGAA	SPSAAEKGEP	AAAAAPEAGA
110	120	130	140	150
SPVEKEAPAE	GEAAEPGSPT	AAEGEAASAA	SSTSSPKAED	GATPSPSNET
160	170	180	190	200
PKKKKKRFSF	KKSFKLSGFS	FKKNKKEAGE	GGEAEAPAAE	GGKDEAAGGA
210	220	230	240	250
AAAAAEAGAA	SGEQAAAPGE	EAAAGEEGAA	GGDPQEAKPQ	EAAVAPEKPP
260	270	280	290	300
ASDETKAAEE	PSKVEEKKAE	EAGASAAACE	APSAAGPGAP	PEQEAAPAEE
310	320	330	332
PAAAAASSAC	AAPSQEAQPE	CSPEAPPAEA	AE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-976 Collapse assay details

Data source: Panorama

Official Gene Symbol: MARCKS
Peptide Modified Sequence: GEPAAAAAPEAGAS[+80.0]PVEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 101
Peptide - Site of Modification: 14
Peptide Start: 88
Peptide End: 105

CPTAC ID: CPTAC-976
Peptide Molecular Mass: 1,701.7611
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y16 (2+)	28	10.7	11.3	17.3	10.6	12.6	32.9	15.1	16.9	15	24	15
y10 (1+)	22.7	11.8	11.5	22.9	13.6	15.5	32.2	18	19.3	15	24	15
y11 (1+)	29.8	9.7	14.9	39.8	13.5	18	49.7	16.6	23.4	15	24	15
sum	22.9	10.5	11.2	22.3	11.9	14.2	32	15.9	18.1	15	24	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-4045 Collapse assay details

Data source: Panorama

Official Gene Symbol: Marcks
Peptide Sequence: EEPASGSAATPAAAEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 70
Peptide End: 85

CPTAC ID: non-CPTAC-4045
Peptide Molecular Mass: 1,485.6947
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers