Marcks, CPTAC-4045 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Marcks	Macs

Sequence Length (AA)	Molecular Weight (Da)
309	29661

Protein Name
Myristoylated alanine-rich C-kinase substrate

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAQFSKTAA	KGEATAERPG	EAAVASSPSK	ANGQENGHVK	VNGDASPAAA
60	70	80	90	100
EPGAKEELQA	NGSAPAADKE	EPASGSAATP	AAAEKDEAAA	ATEPGAGAAD
110	120	130	140	150
KEAAEAEPAE	PSSPAAEAEG	ASASSTSSPK	AEDGAAPSPS	SETPKKKKKR
160	170	180	190	200
FSFKKSFKLS	GFSFKKSKKE	SGEGAEAEGA	TAEGAKDEAA	AAAGGEGAAA
210	220	230	240	250
PGEQAGGAGA	EGAAGGEPRE	AEAAEPEQPE	QPEQPAAEEP	QAEEQSEAAG
260	270	280	290	300
EKAEEPAPGA	TAGDASSAAG	PEQEAPAATD	EAAASAAPAA	SPEPQPECSP
309
EAPPAPTAE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-976 Collapse assay details

Data source: Panorama

Official Gene Symbol: MARCKS
Peptide Modified Sequence: GEPAAAAAPEAGAS[+80.0]PVEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 101
Peptide - Site of Modification: 14
Peptide Start: 88
Peptide End: 105

CPTAC ID: CPTAC-976
Peptide Molecular Mass: 1,701.7611
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y16 (2+)	28	10.7	11.3	17.3	10.6	12.6	32.9	15.1	16.9	15	24	15
y10 (1+)	22.7	11.8	11.5	22.9	13.6	15.5	32.2	18	19.3	15	24	15
y11 (1+)	29.8	9.7	14.9	39.8	13.5	18	49.7	16.6	23.4	15	24	15
sum	22.9	10.5	11.2	22.3	11.9	14.2	32	15.9	18.1	15	24	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-4045 Collapse assay details

Data source: Panorama

Official Gene Symbol: Marcks
Peptide Sequence: EEPASGSAATPAAAEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 70
Peptide End: 85

CPTAC ID: non-CPTAC-4045
Peptide Molecular Mass: 1,485.6947
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers