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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Lepr Db, Obr
Sequence Length (AA) Molecular Weight (Da)
1162 130789
Protein Name
Leptin receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMCQKFYVVL LHWEFLYVIA ALNLAYPISP WKFKLFCGPP NTTDDSFLSP
60 70 80 90 100
AGAPNNASAL KGASEAIVEA KFNSSGIYVP ELSKTVFHCC FGNEQGQNCS
110 120 130 140 150
ALTDNTEGKT LASVVKASVF RQLGVNWDIE CWMKGDLTLF ICHMEPLPKN
160 170 180 190 200
PFKNYDSKVH LLYDLPEVID DSPLPPLKDS FQTVQCNCSL RGCECHVPVP
210 220 230 240 250
RAKLNYALLM YLEITSAGVS FQSPLMSLQP MLVVKPDPPL GLHMEVTDDG
260 270 280 290 300
NLKISWDSQT MAPFPLQYQV KYLENSTIVR EAAEIVSATS LLVDSVLPGS
310 320 330 340 350
SYEVQVRSKR LDGSGVWSDW SSPQVFTTQD VVYFPPKILT SVGSNASFHC
360 370 380 390 400
IYKNENQIIS SKQIVWWRNL AEKIPEIQYS IVSDRVSKVT FSNLKATRPR
410 420 430 440 450
GKFTYDAVYC CNEQACHHRY AELYVIDVNI NISCETDGYL TKMTCRWSPS
460 470 480 490 500
TIQSLVGSTV QLRYHRRSLY CPDSPSIHPT SEPKNCVLQR DGFYECVFQP
510 520 530 540 550
IFLLSGYTMW IRINHSLGSL DSPPTCVLPD SVVKPLPPSN VKAEITVNTG
560 570 580 590 600
LLKVSWEKPV FPENNLQFQI RYGLSGKEIQ WKTHEVFDAK SKSASLLVSD
610 620 630 640 650
LCAVYVVQVR CRRLDGLGYW SNWSSPAYTL VMDVKVPMRG PEFWRKMDGD
660 670 680 690 700
VTKKERNVTL LWKPLTKNDS LCSVRRYVVK HRTAHNGTWS EDVGNRTNLT
710 720 730 740 750
FLWTEPAHTV TVLAVNSLGA SLVNFNLTFS WPMSKVSAVE SLSAYPLSSS
760 770 780 790 800
CVILSWTLSP DDYSLLYLVI EWKILNEDDG MKWLRIPSNV KKFYIHDNFI
810 820 830 840 850
PIEKYQFSLY PVFMEGVGKP KIINGFTKDA IDKQQNDAGL YVIVPIIISS
860 870 880 890 900
CVLLLGTLLI SHQRMKKLFW DDVPNPKNCS WAQGLNFQKP ETFEHLFTKH
910 920 930 940 950
AESVIFGPLL LEPEPISEEI SVDTAWKNKD EMVPAAMVSL LLTTPDPESS
960 970 980 990 1000
SICISDQCNS ANFSGSQSTQ VTCEDECQRQ PSVKYATLVS NDKLVETDEE
1010 1020 1030 1040 1050
QGFIHSPVSN CISSNHSPLR QSFSSSSWET EAQTFFLLSD QQPTMISPQL
1060 1070 1080 1090 1100
SFSGLDELLE LEGSFPEENH REKSVCYLGV TSVNRRESGV LLTGEAGILC
1110 1120 1130 1140 1150
TFPAQCLFSD IRILQERCSH FVENNLSLGT SGENFVPYMP QFQTCSTHSH
1160 1162
KIMENKMCDL TV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4044 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lepr
Peptide Sequence
VTFSNLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
389
Peptide End
395
CPTAC ID
non-CPTAC-4044
Peptide Molecular Mass
807.4491
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 11.2 9.3 4.9 14.6 10.3 8.5 18.4 13.9 9.8 15 15 15
y5 (1+) 9.2 4.4 5.1 9.3 6.2 5.5 13.1 7.6 7.5 15 15 15
y4 (1+) 6.3 7.9 6.1 16.1 8.4 7.9 17.3 11.5 10 15 15 15
y2 (1+) 7.8 6.4 4.6 15.5 10 8.1 17.4 11.9 9.3 15 15 15
b2 (1+) 5.5 7.8 3 8.8 7.6 8.1 10.4 10.9 8.6 15 15 15
sum 10.1 4.3 3.5 6.9 6.4 6.2 12.2 7.7 7.1 15 15 15

Additional Resources and Comments