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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Megf9

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Megf9 Egfl5, Kiaa0818
Sequence Length (AA) Molecular Weight (Da)
600 62831
Protein Name
Multiple epidermal growth factor-like domains protein 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNGGAERAMR SLPSLGGLAL LCCAAAAAAS TASAGNVTGG GGAEGQVVPS
60 70 80 90 100
PSPGLRDQAS SPFPKTAAPT AQAPRTGPPR TTVRKTGATT PSAGSPEIIP
110 120 130 140 150
PLRTSAQPAA TPFPALDLSP ATPSEDGHTP TTESPPSRPA PTTLASTVGQ
160 170 180 190 200
PPTTSVVTTA QASSTPGTPT AESPDRSSNS SGVPPTAPVT EAPTSPPPEH
210 220 230 240 250
MCNCSEVGSL DVKRCNQTTG QCDCHVGYQG LHCDTCKEGF YLNHTVGLCL
260 270 280 290 300
PCHCSPHGAV SILCNSSGNC QCKVGVTGSM CDKCQDGHYG FGKTGCLPCQ
310 320 330 340 350
CNNRSDSCDV HTGACLNCQE NSKGEHCEEC KEGFYPSPDA AKQCHRCPCS
360 370 380 390 400
AVTSTGNCTI ESGELEPTCD QCKDGYTGQN CNKCENGYYN SDSICTQCEC
410 420 430 440 450
HGHVDPIKTP KICKPESGEC INCLHNTTGF WCEKCLEGYV RDLQRNCIKQ
460 470 480 490 500
EVIVPTPEGS TILVSNASLT TSVPTPVINS TFAPTTLQTI FAVSSSENST
510 520 530 540 550
SALADVSWTQ FNIIILTVII IVVVLLMGFV GAVYMYREYQ NRKLNAPFWT
560 570 580 590 600
IELKEDNISF SSYHDSIPNA DVSGLLEDDA NEVAPNGQLT LTTPIHNYKA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4046 Collapse assay details

Data source: Panorama

Official Gene Symbol
Megf9
Peptide Sequence
EGFYPSPDAAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
332
Peptide End
342
CPTAC ID
non-CPTAC-4046
Peptide Molecular Mass
1,180.5401
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 27.2 12.8 11.8 24.5 13.3 12.6 36.6 18.5 17.3 15 15 15
y7 (2+) 37.5 13.4 5.2 34.8 11.9 8.5 51.2 17.9 10 15 15 15
b3 (1+) 13.3 10.4 8.4 13.9 10.6 9.6 19.2 14.8 12.8 15 15 15
y8 (1+) 30.2 10.9 7.1 42.6 15.3 10.6 52.2 18.8 12.8 15 15 15
y7 (1+) 8.4 7.8 6.3 16.7 7 9.3 18.7 10.5 11.2 15 15 15
sum 6.1 4.4 3.5 13.6 6.7 6.8 14.9 8 7.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments