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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mmp9

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mmp9 Clg4b
Sequence Length (AA) Molecular Weight (Da)
730 80535
Protein Name
Matrix metalloproteinase-9
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSPWQPLLLA LLAFGCSSAA PYQRQPTFVV FPKDLKTSNL TDTQLAEAYL
60 70 80 90 100
YRYGYTRAAQ MMGEKQSLRP ALLMLQKQLS LPQTGELDSQ TLKAIRTPRC
110 120 130 140 150
GVPDVGRFQT FKGLKWDHHN ITYWIQNYSE DLPRDMIDDA FARAFAVWGE
160 170 180 190 200
VAPLTFTRVY GPEADIVIQF GVAEHGDGYP FDGKDGLLAH AFPPGAGVQG
210 220 230 240 250
DAHFDDDELW SLGKGVVIPT YYGNSNGAPC HFPFTFEGRS YSACTTDGRN
260 270 280 290 300
DGTPWCSTTA DYDKDGKFGF CPSERLYTEH GNGEGKPCVF PFIFEGRSYS
310 320 330 340 350
ACTTKGRSDG YRWCATTANY DQDKLYGFCP TRVDATVVGG NSAGELCVFP
360 370 380 390 400
FVFLGKQYSS CTSDGRRDGR LWCATTSNFD TDKKWGFCPD QGYSLFLVAA
410 420 430 440 450
HEFGHALGLD HSSVPEALMY PLYSYLEGFP LNKDDIDGIQ YLYGRGSKPD
460 470 480 490 500
PRPPATTTTE PQPTAPPTMC PTIPPTAYPT VGPTVGPTGA PSPGPTSSPS
510 520 530 540 550
PGPTGAPSPG PTAPPTAGSS EASTESLSPA DNPCNVDVFD AIAEIQGALH
560 570 580 590 600
FFKDGWYWKF LNHRGSPLQG PFLTARTWPA LPATLDSAFE DPQTKRVFFF
610 620 630 640 650
SGRQMWVYTG KTVLGPRSLD KLGLGPEVTH VSGLLPRRLG KALLFSKGRV
660 670 680 690 700
WRFDLKSQKV DPQSVIRVDK EFSGVPWNSH DIFQYQDKAY FCHGKFFWRV
710 720 730
SFQNEVNKVD HEVNQVDDVG YVTYDLLQCP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4047 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mmp9
Peptide Sequence
VSFQNEVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
700
Peptide End
708
CPTAC ID
non-CPTAC-4047
Peptide Molecular Mass
1,063.5298
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 9.1 8.2 7.2 11.6 11.3 11.4 14.7 14 13.5 14 15 15
y2 (1+) 19.6 7.5 15.6 21.1 7 17.5 28.8 10.3 23.4 14 15 15
y6 (1+) 13.2 11.4 8.3 14.5 10.1 10.7 19.6 15.2 13.5 14 15 15
y7 (1+) 6.4 11.6 8.7 8.9 12 11.1 11 16.7 14.1 14 15 15
y8 (1+) 8.2 9.2 5.2 12.4 12 8.4 14.9 15.1 9.9 14 15 15
sum 3.7 6.8 5.8 7.1 9.4 9.8 8 11.6 11.4 14 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments