MOCOS, CPTAC-979 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MOCOS	N/A

Sequence Length (AA)	Molecular Weight (Da)
888	98120

Protein Name
Molybdenum cofactor sulfurase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGAAAESGR	ELWTFAGSRD	PSAPRLAYGY	GPGSLRELRA	REFSRLAGTV
60	70	80	90	100
YLDHAGATLF	SQSQLESFTS	DLMENTYGNP	HSQNISSKLT	HDTVEQVRYR
110	120	130	140	150
ILAHFHTTAE	DYTVIFTAGS	TAALKLVAEA	FPWVSQGPES	SGSRFCYLTD
160	170	180	190	200
SHTSVVGMRN	VTMAINVIST	PVRPEDLWSA	EERSASASNP	DCQLPHLFCY
210	220	230	240	250
PAQSNFSGVR	YPLSWIEEVK	SGRLHPVSTP	GKWFVLLDAA	SYVSTSPLDL
260	270	280	290	300
SAHQADFVPI	SFYKIFGFPT	GLGALLVHNR	AAPLLRKTYF	GGGTASAYLA
310	320	330	340	350
GEDFYIPRQS	VAQRFEDGTI	SFLDVIALKH	GFDTLERLTG	GMENIKQHTF
360	370	380	390	400
TLAQYTYVAL	SSLQYPNGAP	VVRIYSDSEF	SSPEVQGPII	NFNVLDDKGN
410	420	430	440	450
IIGYSQVDKM	ASLYNIHLRT	GCFCNTGACQ	RHLGISNEMV	RKHFQAGHVC
460	470	480	490	500
GDNMDLIDGQ	PTGSVRISFG	YMSTLDDVQA	FLRFIIDTRL	HSSGDWPVPQ
510	520	530	540	550
AHADTGETGA	PSADSQADVI	PAVMGRRSLS	PQEDALTGSR	VWNNSSTVNA
560	570	580	590	600
VPVAPPVCDV	ARTQPTPSEK	AAGVLEGALG	PHVVTNLYLY	PIKSCAAFEV
610	620	630	640	650
TRWPVGNQGL	LYDRSWMVVN	HNGVCLSQKQ	EPRLCLIQPF	IDLRQRIMVI
660	670	680	690	700
KAKGMEPIEV	PLEENSERTQ	IRQSRVCADR	VSTYDCGEKI	SSWLSTFFGR
710	720	730	740	750
PCHLIKQSSN	SQRNAKKKHG	KDQLPGTMAT	LSLVNEAQYL	LINTSSILEL
760	770	780	790	800
HRQLNTSDEN	GKEELFSLKD	LSLRFRANII	INGKRAFEEE	KWDEISIGSL
810	820	830	840	850
RFQVLGPCHR	CQMICIDQQT	GQRNQHVFQK	LSESRETKVN	FGMYLMHASL
860	870	880	888
DLSSPCFLSV	GSQVLPVLKE	NVEGHDLPAS	EKHQDVTS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-979 Collapse assay details

Data source: Panorama

Official Gene Symbol: MOCOS
Peptide Modified Sequence: SLS[+80.0]PQEDALTGSR
Modification Type: Phospho (ST)
Protein - Site of Modification: 530
Peptide - Site of Modification: 3
Peptide Start: 528
Peptide End: 540

CPTAC ID: CPTAC-979
Peptide Molecular Mass: 1,439.6294
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	24	7.3	6.6	21.6	8.8	14	32.3	11.4	15.5	15	24	15
y11 (2+)	37.9	12.5	27.5	33.1	11.7	36.2	50.3	17.1	45.5	15	24	15
y10 (1+)	23.9	8.6	9.7	19.3	10.6	9.6	30.7	13.6	13.6	15	24	15
sum	21.5	5.7	7.3	19.5	7.3	12.9	29	9.3	14.8	15	24	15

MOCOS

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-979 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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MOCOS

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-979 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments