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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MYBBP1A P160
Sequence Length (AA) Molecular Weight (Da)
1328 148855
Protein Name
Myb-binding protein 1A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESRDPAQPM SPGEATQSGA RPADRYGLLK HSREFLDFFW DIAKPEQETR
60 70 80 90 100
LAATEKLLEY LRGRPKGSEM KYALKRLITG LGVGRETARP CYSLALAQLL
110 120 130 140 150
QSFEDLPLCS ILQQIQEKYD LHQVKKAMLR PALFANLFGV LALFQSGRLV
160 170 180 190 200
KDQEALMKSV KLLQALAQYQ NHLQEQPRKA LVDILSEVSK ATLQEILPEV
210 220 230 240 250
LKADLNIILS SPEQLELFLL AQQKVPSKLK KLVGSVNLFS DENVPRLVNV
260 270 280 290 300
LKMAASSVKK DRKLPAIALD LLRLALKEDK FPRFWKEVVE QGLLKMQFWP
310 320 330 340 350
ASYLCFRLLG AALPLLTKEQ LHLVMQGDVI RHYGEHVCTA KLPKQFKFAP
360 370 380 390 400
EMDDYVGTFL EGCQDDPERQ LAVLVAFSSV TNQGLPVTPT FWRVVRFLSP
410 420 430 440 450
PALQGYVAWL RAMFLQPDLD SLVDFSTNNQ KKAQDSSLHM PERAVFRLRK
460 470 480 490 500
WIIFRLVSIV DSLHLEMEEA LTEQVARFCL FHSFFVTKKP TSQIPETKHP
510 520 530 540 550
FSFPLENQAR EAVSSAFFSL LQTLSTQFKQ APGQTQGGQP WTYHLVQFAD
560 570 580 590 600
LLLNHSHNVT TVTPFTAQQR QAWDRMLQTL KELEAHSAEA RAAAFQHLLL
610 620 630 640 650
LVGIHLLKSP AESCDLLGDI QTCIRKSLGE KPRRSRTKTI DPQEPPWVEV
660 670 680 690 700
LVEILLALLA QPSHLMRQVA RSVFGHICSH LTPRALQLIL DVLNPETSED
710 720 730 740 750
ENDRVVVTDD SDERRLKGAE DKSEEGEDNR SSESEEESEG EESEEEERDG
760 770 780 790 800
DVDQGFREQL MTVLQAGKAL GGEDSENEEE LGDEAMMALD QSLASLFAEQ
810 820 830 840 850
KLRIQARRDE KNKLQKEKAL RRDFQIRVLD LVEVLVTKQP ENALVLELLE
860 870 880 890 900
PLLSIIRRSL RSSSSKQEQD LLHKTARIFT HHLCRARRYC HDLGERAGAL
910 920 930 940 950
HAQVERLVQQ AGRQPDSPTA LYHFNASLYL LRVLKGNTAE GCVHETQEKQ
960 970 980 990 1000
KAGTDPSHMP TGPQAASCLD LNLVTRVYST ALSSFLTKRN SPLTVPMFLS
1010 1020 1030 1040 1050
LFSRHPVLCQ SLLPILVQHI TGPVRPRHQA CLLLQKTLSM REVRSCFEDP
1060 1070 1080 1090 1100
EWKQLMGQVL AKVTENLRVL GEAQTKAQHQ QALSSLELLN VLFRTCKHEK
1110 1120 1130 1140 1150
LTLDLTVLLG VLQGQQQSLQ QGAHSTGSSR LHDLYWQAMK TLGVQRPKLE
1160 1170 1180 1190 1200
KKDAKEIPSA TQSPISKKRK KKGFLPETKK RKKRKSEDGT PAEDGTPAAT
1210 1220 1230 1240 1250
GGSQPPSMGR KKRNRTKAKV PAQANGTPTT KSPAPGAPTR SPSTPAKSPK
1260 1270 1280 1290 1300
LQKKNQKPSQ VNGAPGSPTE PAGQKQHQKA LPKKGVLGKS PLSALARKKA
1310 1320 1328
RLSLVIRSPS LLQSGAKKKA QVRKAGKP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-980 Collapse assay details

Data source: Panorama

Official Gene Symbol
MYBBP1A
Peptide Modified Sequence
EIPSATQS[+80.0]PISK
Modification Type
Phospho (ST)
Protein - Site of Modification
1163
Peptide - Site of Modification
8
Peptide Start
1156
Peptide End
1167
CPTAC ID
CPTAC-980
Peptide Molecular Mass
1,336.6276
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 13.7 7.8 4.3 23.7 7.9 7 27.4 11.1 8.2 15 24 15
y7 (1+) 18 8.4 8.2 26.2 9.4 9.7 31.8 12.6 12.7 15 24 15
y10 (1+) 14.2 8.7 8.3 23.9 8.7 9.9 27.8 12.3 12.9 15 24 15
sum 13.7 7.8 4.3 23.7 8 6.2 27.4 11.2 7.5 15 24 15

Additional Resources and Comments