MYBBP1A, CPTAC-980 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MYBBP1A	P160

Sequence Length (AA)	Molecular Weight (Da)
1328	148855

Protein Name
Myb-binding protein 1A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MESRDPAQPM	SPGEATQSGA	RPADRYGLLK	HSREFLDFFW	DIAKPEQETR
60	70	80	90	100
LAATEKLLEY	LRGRPKGSEM	KYALKRLITG	LGVGRETARP	CYSLALAQLL
110	120	130	140	150
QSFEDLPLCS	ILQQIQEKYD	LHQVKKAMLR	PALFANLFGV	LALFQSGRLV
160	170	180	190	200
KDQEALMKSV	KLLQALAQYQ	NHLQEQPRKA	LVDILSEVSK	ATLQEILPEV
210	220	230	240	250
LKADLNIILS	SPEQLELFLL	AQQKVPSKLK	KLVGSVNLFS	DENVPRLVNV
260	270	280	290	300
LKMAASSVKK	DRKLPAIALD	LLRLALKEDK	FPRFWKEVVE	QGLLKMQFWP
310	320	330	340	350
ASYLCFRLLG	AALPLLTKEQ	LHLVMQGDVI	RHYGEHVCTA	KLPKQFKFAP
360	370	380	390	400
EMDDYVGTFL	EGCQDDPERQ	LAVLVAFSSV	TNQGLPVTPT	FWRVVRFLSP
410	420	430	440	450
PALQGYVAWL	RAMFLQPDLD	SLVDFSTNNQ	KKAQDSSLHM	PERAVFRLRK
460	470	480	490	500
WIIFRLVSIV	DSLHLEMEEA	LTEQVARFCL	FHSFFVTKKP	TSQIPETKHP
510	520	530	540	550
FSFPLENQAR	EAVSSAFFSL	LQTLSTQFKQ	APGQTQGGQP	WTYHLVQFAD
560	570	580	590	600
LLLNHSHNVT	TVTPFTAQQR	QAWDRMLQTL	KELEAHSAEA	RAAAFQHLLL
610	620	630	640	650
LVGIHLLKSP	AESCDLLGDI	QTCIRKSLGE	KPRRSRTKTI	DPQEPPWVEV
660	670	680	690	700
LVEILLALLA	QPSHLMRQVA	RSVFGHICSH	LTPRALQLIL	DVLNPETSED
710	720	730	740	750
ENDRVVVTDD	SDERRLKGAE	DKSEEGEDNR	SSESEEESEG	EESEEEERDG
760	770	780	790	800
DVDQGFREQL	MTVLQAGKAL	GGEDSENEEE	LGDEAMMALD	QSLASLFAEQ
810	820	830	840	850
KLRIQARRDE	KNKLQKEKAL	RRDFQIRVLD	LVEVLVTKQP	ENALVLELLE
860	870	880	890	900
PLLSIIRRSL	RSSSSKQEQD	LLHKTARIFT	HHLCRARRYC	HDLGERAGAL
910	920	930	940	950
HAQVERLVQQ	AGRQPDSPTA	LYHFNASLYL	LRVLKGNTAE	GCVHETQEKQ
960	970	980	990	1000
KAGTDPSHMP	TGPQAASCLD	LNLVTRVYST	ALSSFLTKRN	SPLTVPMFLS
1010	1020	1030	1040	1050
LFSRHPVLCQ	SLLPILVQHI	TGPVRPRHQA	CLLLQKTLSM	REVRSCFEDP
1060	1070	1080	1090	1100
EWKQLMGQVL	AKVTENLRVL	GEAQTKAQHQ	QALSSLELLN	VLFRTCKHEK
1110	1120	1130	1140	1150
LTLDLTVLLG	VLQGQQQSLQ	QGAHSTGSSR	LHDLYWQAMK	TLGVQRPKLE
1160	1170	1180	1190	1200
KKDAKEIPSA	TQSPISKKRK	KKGFLPETKK	RKKRKSEDGT	PAEDGTPAAT
1210	1220	1230	1240	1250
GGSQPPSMGR	KKRNRTKAKV	PAQANGTPTT	KSPAPGAPTR	SPSTPAKSPK
1260	1270	1280	1290	1300
LQKKNQKPSQ	VNGAPGSPTE	PAGQKQHQKA	LPKKGVLGKS	PLSALARKKA
1310	1320	1328
RLSLVIRSPS	LLQSGAKKKA	QVRKAGKP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-980 Collapse assay details

Data source: Panorama

Official Gene Symbol: MYBBP1A
Peptide Modified Sequence: EIPSATQS[+80.0]PISK
Modification Type: Phospho (ST)
Protein - Site of Modification: 1163
Peptide - Site of Modification: 8
Peptide Start: 1156
Peptide End: 1167

CPTAC ID: CPTAC-980
Peptide Molecular Mass: 1,336.6276
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	13.7	7.8	4.3	23.7	7.9	7	27.4	11.1	8.2	15	24	15
y7 (1+)	18	8.4	8.2	26.2	9.4	9.7	31.8	12.6	12.7	15	24	15
y10 (1+)	14.2	8.7	8.3	23.9	8.7	9.9	27.8	12.3	12.9	15	24	15
sum	13.7	7.8	4.3	23.7	8	6.2	27.4	11.2	7.5	15	24	15

MYBBP1A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-980 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

MYBBP1A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-980 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments