NFATC1, CPTAC-983 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NFATC1	NFAT2, NFATC

Sequence Length (AA)	Molecular Weight (Da)
943	101243

Protein Name
Nuclear factor of activated T-cells, cytoplasmic 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPSTSFPVPS	KFPLGPAAAV	FGRGETLGPA	PRAGGTMKSA	EEEHYGYASS
60	70	80	90	100
NVSPALPLPT	AHSTLPAPCH	NLQTSTPGII	PPADHPSGYG	AALDGGPAGY
110	120	130	140	150
FLSSGHTRPD	GAPALESPRI	EITSCLGLYH	NNNQFFHDVE	VEDVLPSSKR
160	170	180	190	200
SPSTATLSLP	SLEAYRDPSC	LSPASSLSSR	SCNSEASSYE	SNYSYPYASP
210	220	230	240	250
QTSPWQSPCV	SPKTTDPEEG	FPRGLGACTL	LGSPRHSPST	SPRASVTEES
260	270	280	290	300
WLGARSSRPA	SPCNKRKYSL	NGRQPPYSPH	HSPTPSPHGS	PRVSVTDDSW
310	320	330	340	350
LGNTTQYTSS	AIVAAINALT	TDSSLDLGDG	VPVKSRKTTL	EQPPSVALKV
360	370	380	390	400
EPVGEDLGSP	PPPADFAPED	YSSFQHIRKG	GFCDQYLAVP	QHPYQWAKPK
410	420	430	440	450
PLSPTSYMSP	TLPALDWQLP	SHSGPYELRI	EVQPKSHHRA	HYETEGSRGA
460	470	480	490	500
VKASAGGHPI	VQLHGYLENE	PLMLQLFIGT	ADDRLLRPHA	FYQVHRITGK
510	520	530	540	550
TVSTTSHEAI	LSNTKVLEIP	LLPENSMRAV	IDCAGILKLR	NSDIELRKGE
560	570	580	590	600
TDIGRKNTRV	RLVFRVHVPQ	PSGRTLSLQV	ASNPIECSQR	SAQELPLVEK
610	620	630	640	650
QSTDSYPVVG	GKKMVLSGHN	FLQDSKVIFV	EKAPDGHHVW	EMEAKTDRDL
660	670	680	690	700
CKPNSLVVEI	PPFRNQRITS	PVHVSFYVCN	GKRKRSQYQR	FTYLPANVPI
710	720	730	740	750
IKTEPTDDYE	PAPTCGPVSQ	GLSPLPRPYY	SQQLAMPPDP	SSCLVAGFPP
760	770	780	790	800
CPQRSTLMPA	APGVSPKLHD	LSPAAYTKGV	ASPGHCHLGL	PQPAGEAPAV
810	820	830	840	850
QDVPRPVATH	PGSPGQPPPA	LLPQQVSAPP	SSSCPPGLEH	SLCPSSPSPP
860	870	880	890	900
LPPATQEPTC	LQPCSPACPP	ATGRPQHLPS	TVRRDESPTA	GPRLLPEVHE
910	920	930	940	943
DGSPNLAPIP	VTVKREPEEL	DQLYLDDVNE	IIRNDLSSTS	THS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-983 Collapse assay details

Data source: Panorama

Official Gene Symbol: NFATC1
Peptide Modified Sequence: GLGAC[+57.0]TLLGS[+80.0]PR
Modification Type: Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification: 228, 233
Peptide - Site of Modification: 5, 10
Peptide Start: 224
Peptide End: 235

CPTAC ID: CPTAC-983
Peptide Molecular Mass: 1,280.5948
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b8 (1+)	33.8	23.8	42.4	30.3	23.3	43.5	45.4	33.3	60.7	15	24	15
y4 (1+)	29.9	11.3	22.7	19.6	10.2	20.6	35.8	15.2	30.7	15	24	15
y5 (1+)	34.9	23.5	21	28.5	36.2	26.8	45.1	43.2	34	15	24	15
sum	29.3	13.8	11	17.9	18.1	14	34.3	22.8	17.8	15	24	15

NFATC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-983 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NFATC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-983 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments