NSUN2, CPTAC-984 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NSUN2	SAKI, TRM4

Sequence Length (AA)	Molecular Weight (Da)
767	86471

Protein Name
tRNA (cytosine(34)-C(5))-methyltransferase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGRRSRGRRL	QQQQRPEDAE	DGAEGGGKRG	EAGWEGGYPE	IVKENKLFEH
60	70	80	90	100
YYQELKIVPE	GEWGQFMDAL	REPLPATLRI	TGYKSHAKEI	LHCLKNKYFK
110	120	130	140	150
ELEDLEVDGQ	KVEVPQPLSW	YPEELAWHTN	LSRKILRKSP	HLEKFHQFLV
160	170	180	190	200
SETESGNISR	QEAVSMIPPL	LLNVRPHHKI	LDMCAAPGSK	TTQLIEMLHA
210	220	230	240	250
DMNVPFPEGF	VIANDVDNKR	CYLLVHQAKR	LSSPCIMVVN	HDASSIPRLQ
260	270	280	290	300
IDVDGRKEIL	FYDRILCDVP	CSGDGTMRKN	IDVWKKWTTL	NSLQLHGLQL
310	320	330	340	350
RIATRGAEQL	AEGGRMVYST	CSLNPIEDEA	VIASLLEKSE	GALELADVSN
360	370	380	390	400
ELPGLKWMPG	ITQWKVMTKD	GQWFTDWDAV	PHSRHTQIRP	TMFPPKDPEK
410	420	430	440	450
LQAMHLERCL	RILPHHQNTG	GFFVAVLVKK	SSMPWNKRQP	KLQGKSAETR
460	470	480	490	500
ESTQLSPADL	TEGKPTDPSK	LESPSFTGTG	DTEIAHATED	LENNGSKKDG
510	520	530	540	550
VCGPPPSKKM	KLFGFKEDPF	VFIPEDDPLF	PPIEKFYALD	PSFPRMNLLT
560	570	580	590	600
RTTEGKKRQL	YMVSKELRNV	LLNNSEKMKV	INTGIKVWCR	NNSGEEFDCA
610	620	630	640	650
FRLAQEGIYT	LYPFINSRII	TVSMEDVKIL	LTQENPFFRK	LSSETYSQAK
660	670	680	690	700
DLAKGSIVLK	YEPDSANPDA	LQCPIVLCGW	RGKASIRTFV	PKNERLHYLR
710	720	730	740	750
MMGLEVLGEK	KKEGVILTNE	SAASTGQPDN	DVTEGQRAGE	PNSPDAEEAN
760	767
SPDVTAGCDP	AGVHPPR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-984 Collapse assay details

Data source: Panorama

Official Gene Symbol: NSUN2
Peptide Modified Sequence: ESTQLS[+80.0]PADLTEGKPTDPSK
Modification Type: Phospho (ST)
Protein - Site of Modification: 456
Peptide - Site of Modification: 6
Peptide Start: 451
Peptide End: 470

CPTAC ID: CPTAC-984
Peptide Molecular Mass: 2,179.9886
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y15 (2+)	33.8	12.7	13.3	23.2	13.8	14.7	41	18.8	19.8	15	24	15
y16-98 (2+)	30.3	13.7	22.5	22.6	19.4	17	37.8	23.7	28.2	15	24	15
y16 (2+)	29.2	11.8	19.3	20.9	16.6	21.7	35.9	20.4	29	15	24	15
sum	27.5	10.9	11.9	19.4	14.1	13.5	33.7	17.8	18	15	24	15

NSUN2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-984 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NSUN2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-984 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments