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Overview Data source: UniProt

Official Gene Symbol Other Aliases
NUFIP2 KIAA1321
Sequence Length (AA) Molecular Weight (Da)
695 76121
Protein Name
Nuclear fragile X mental retardation-interacting protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEEKPGQPQP QHHHSHHHPH HHPQQQQQQP HHHHHYYFYN HSHNHHHHHH
60 70 80 90 100
HQQPHQYLQH GAEGSPKAQP KPLKHEQKHT LQQHQETPKK KTGYGELNGN
110 120 130 140 150
AGEREISLKN LSSDEATNPI SRVLNGNQQV VDTSLKQTVK ANTFGKAGIK
160 170 180 190 200
TKNFIQKNSM DKKNGKSYEN KSGENQSVDK SDTIPIPNGV VTNNSGYITN
210 220 230 240 250
GYMGKGADND GSGSESGYTT PKKRKARRNS AKGCENLNIV QDKIMQQETS
260 270 280 290 300
VPTLKQGLET FKPDYSEQKG NRVDGSKPIW KYETGPGGTS RGKPAVGDML
310 320 330 340 350
RKSSDSKPGV SSKKFDDRPK GKHASAVASK EDSWTLFKPP PVFPVDNSSA
360 370 380 390 400
KIVPKISYAS KVKENLNKTI QNSSVSPTSS SSSSSSTGET QTQSSSRLSQ
410 420 430 440 450
VPMSALKSVT SANFSNGPVL AGTDGNVYPP GGQPLLTTAA NTLTPISSGT
460 470 480 490 500
DSVLQDMSLT SAAVEQIKTS LFIYPSNMQT MLLSTAQVDL PSQTDQQNLG
510 520 530 540 550
DIFQNQWGLS FINEPSAGPE TVTGKSSEHK VMEVTFQGEY PATLVSQGAE
560 570 580 590 600
IIPSGTEHPV FPKAYELEKR TSPQVLGSIL KSGTTSESGA LSLEPSHIGD
610 620 630 640 650
LQKADTSSQG ALVFLSKDYE IESQNPLASP TNTLLGSAKE QRYQRGLERN
660 670 680 690 695
DSWGSFDLRA AIVYHTKEME SIWNLQKQDP KRIITYNEAM DSPDQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-985 Collapse assay details

Data source: Panorama

Official Gene Symbol
NUFIP2
Peptide Modified Sequence
NDS[+80.0]WGSFDLR
Modification Type
Phospho (ST)
Protein - Site of Modification
652
Peptide - Site of Modification
3
Peptide Start
650
Peptide End
659
CPTAC ID
CPTAC-985
Peptide Molecular Mass
1,275.4921
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 26.2 7.8 7.6 22.3 11.8 9.6 34.4 14.1 12.2 15 24 15
y7 (1+) 33.9 9.2 5.2 16.5 12.6 7.2 37.7 15.6 8.9 15 24 15
y8 (1+) 55.1 18 27.4 48.1 18.7 29.7 73.1 26 40.4 15 24 15
sum 30.8 7.4 7.3 19.5 12 9.8 36.5 14.1 12.2 15 24 15

Additional Resources and Comments