NUFIP2, CPTAC-985 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NUFIP2	KIAA1321

Sequence Length (AA)	Molecular Weight (Da)
695	76121

Protein Name
Nuclear fragile X mental retardation-interacting protein 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEEKPGQPQP	QHHHSHHHPH	HHPQQQQQQP	HHHHHYYFYN	HSHNHHHHHH
60	70	80	90	100
HQQPHQYLQH	GAEGSPKAQP	KPLKHEQKHT	LQQHQETPKK	KTGYGELNGN
110	120	130	140	150
AGEREISLKN	LSSDEATNPI	SRVLNGNQQV	VDTSLKQTVK	ANTFGKAGIK
160	170	180	190	200
TKNFIQKNSM	DKKNGKSYEN	KSGENQSVDK	SDTIPIPNGV	VTNNSGYITN
210	220	230	240	250
GYMGKGADND	GSGSESGYTT	PKKRKARRNS	AKGCENLNIV	QDKIMQQETS
260	270	280	290	300
VPTLKQGLET	FKPDYSEQKG	NRVDGSKPIW	KYETGPGGTS	RGKPAVGDML
310	320	330	340	350
RKSSDSKPGV	SSKKFDDRPK	GKHASAVASK	EDSWTLFKPP	PVFPVDNSSA
360	370	380	390	400
KIVPKISYAS	KVKENLNKTI	QNSSVSPTSS	SSSSSSTGET	QTQSSSRLSQ
410	420	430	440	450
VPMSALKSVT	SANFSNGPVL	AGTDGNVYPP	GGQPLLTTAA	NTLTPISSGT
460	470	480	490	500
DSVLQDMSLT	SAAVEQIKTS	LFIYPSNMQT	MLLSTAQVDL	PSQTDQQNLG
510	520	530	540	550
DIFQNQWGLS	FINEPSAGPE	TVTGKSSEHK	VMEVTFQGEY	PATLVSQGAE
560	570	580	590	600
IIPSGTEHPV	FPKAYELEKR	TSPQVLGSIL	KSGTTSESGA	LSLEPSHIGD
610	620	630	640	650
LQKADTSSQG	ALVFLSKDYE	IESQNPLASP	TNTLLGSAKE	QRYQRGLERN
660	670	680	690	695
DSWGSFDLRA	AIVYHTKEME	SIWNLQKQDP	KRIITYNEAM	DSPDQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-985 Collapse assay details

Data source: Panorama

Official Gene Symbol: NUFIP2
Peptide Modified Sequence: NDS[+80.0]WGSFDLR
Modification Type: Phospho (ST)
Protein - Site of Modification: 652
Peptide - Site of Modification: 3
Peptide Start: 650
Peptide End: 659

CPTAC ID: CPTAC-985
Peptide Molecular Mass: 1,275.4921
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	26.2	7.8	7.6	22.3	11.8	9.6	34.4	14.1	12.2	15	24	15
y7 (1+)	33.9	9.2	5.2	16.5	12.6	7.2	37.7	15.6	8.9	15	24	15
y8 (1+)	55.1	18	27.4	48.1	18.7	29.7	73.1	26	40.4	15	24	15
sum	30.8	7.4	7.3	19.5	12	9.8	36.5	14.1	12.2	15	24	15

NUFIP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-985 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NUFIP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-985 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments