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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PHACTR2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PHACTR2 C6orf56, KIAA0680
Sequence Length (AA) Molecular Weight (Da)
634 69700
Protein Name
Phosphatase and actin regulator 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDNAVDGLDK ASIANSDGPT AGSQTPPFKR KGKLSTIGKI FKPWKWRKKK
60 70 80 90 100
TSDKFRETSA VLERKISTRQ SREELIRRGV LKELPDQDGD VTVNFENSNG
110 120 130 140 150
HMIPIGEEST REENVVKSEE GNGSVSEKTP PLEEQAEDKK ENTENHSETP
160 170 180 190 200
AAPALPPSAP PKPRPKPKPK KSPVPPKGAT AGASHKGDEV PPIKKNTKAP
210 220 230 240 250
GKQAPVPPPK PASRNTTREA AGSSHSKKTT GSKASASPST SSTSSRPKAS
260 270 280 290 300
KETVSSKAGT VGTTKGKRKT DKQPITSHLS SDTTTSGTSD LKGEPAETRV
310 320 330 340 350
ESFKLEQTVP GAEEQNTGKF KSMVPPPPVA PAPSPLAPPL PLEDQCITAS
360 370 380 390 400
DTPVVLVSVG ADLPVSALDP SQLLWAEEPT NRTTLYSGTG LSVNRENAKC
410 420 430 440 450
FTTKEELGKT VPQLLTPGLM GESSESFSAS EDEGHREYQA NDSDSDGPIL
460 470 480 490 500
YTDDEDEDED EDGSGESALA SKIRRRDTLA IKLGNRPSKK ELEDKNILQR
510 520 530 540 550
TSEEERQEIR QQIGTKLVRR LSQRPTTEEL EQRNILKQKN EEEEQEAKME
560 570 580 590 600
LKRRLSRKLS LRPTVAELQA RRILRFNEYV EVTDSPDYDR RADKPWARLT
610 620 630 634
PADKAAIRKE LNEFKSTEME VHEESRQFTR FHRP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-989 Collapse assay details

Data source: Panorama

Official Gene Symbol
PHACTR2
Peptide Modified Sequence
ASIANSDGPTAGSQT[+80.0]PPFK
Modification Type
Phospho (ST)
Protein - Site of Modification
25
Peptide - Site of Modification
15
Peptide Start
11
Peptide End
29
CPTAC ID
CPTAC-989
Peptide Molecular Mass
1,924.8568
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 36 9.9 14.3 27.9 12 18.2 45.5 15.6 23.1 15 24 15
y8 (1+) 30.6 9.5 20.7 35 10.7 23.4 46.5 14.3 31.2 15 24 15
y9 (1+) 29.4 23.7 23.4 37.1 25.8 42.5 47.3 35 48.5 15 24 15
sum 27 7.9 15.1 25.7 10.2 20.8 37.3 12.9 25.7 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments