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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PKP3 N/A
Sequence Length (AA) Molecular Weight (Da)
797 87082
Protein Name
Plakophilin-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQDGNFLLSA LQPEAGVCSL ALPSDLQLDR RGAEGPEAER LRAARVQEQV
60 70 80 90 100
RARLLQLGQQ PRHNGAAEPE PEAETARGTS RGQYHTLQAG FSSRSQGLSG
110 120 130 140 150
DKTSGFRPIA KPAYSPASWS SRSAVDLSCS RRLSSAHNGG SAFGAAGYGG
160 170 180 190 200
AQPTPPMPTR PVSFHERGGV GSRADYDTLS LRSLRLGPGG LDDRYSLVSE
210 220 230 240 250
QLEPAATSTY RAFAYERQAS SSSSRAGGLD WPEATEVSPS RTIRAPAVRT
260 270 280 290 300
LQRFQSSHRS RGVGGAVPGA VLEPVARAPS VRSLSLSLAD SGHLPDVHGF
310 320 330 340 350
NSYGSHRTLQ RLSSGFDDID LPSAVKYLMA SDPNLQVLGA AYIQHKCYSD
360 370 380 390 400
AAAKKQARSL QAVPRLVKLF NHANQEVQRH ATGAMRNLIY DNADNKLALV
410 420 430 440 450
EENGIFELLR TLREQDDELR KNVTGILWNL SSSDHLKDRL ARDTLEQLTD
460 470 480 490 500
LVLSPLSGAG GPPLIQQNAS EAEIFYNATG FLRNLSSASQ ATRQKMRECH
510 520 530 540 550
GLVDALVTSI NHALDAGKCE DKSVENAVCV LRNLSYRLYD EMPPSALQRL
560 570 580 590 600
EGRGRRDLAG APPGEVVGCF TPQSRRLREL PLAADALTFA EVSKDPKGLE
610 620 630 640 650
WLWSPQIVGL YNRLLQRCEL NRHTTEAAAG ALQNITAGDR RWAGVLSRLA
660 670 680 690 700
LEQERILNPL LDRVRTADHH QLRSLTGLIR NLSRNARNKD EMSTKVVSHL
710 720 730 740 750
IEKLPGSVGE KSPPAEVLVN IIAVLNNLVV ASPIAARDLL YFDGLRKLIF
760 770 780 790 797
IKKKRDSPDS EKSSRAASSL LANLWQYNKL HRDFRAKGYR KEDFLGP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-990 Collapse assay details

Data source: Panorama

Official Gene Symbol
PKP3
Peptide Modified Sequence
AGGLDWPEATEVS[+80.0]PSR
Modification Type
Phospho (ST)
Protein - Site of Modification
238
Peptide - Site of Modification
13
Peptide Start
226
Peptide End
241
CPTAC ID
CPTAC-990
Peptide Molecular Mass
1,750.7563
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 29.9 9.5 6.1 14.3 13 6.8 33.1 16.1 9.1 15 24 15
y10-98 (1+) 34.4 10.5 5.9 28.2 14.4 7.7 44.5 17.8 9.7 15 24 15
y10 (1+) 29.3 10.2 6.8 25.9 15.8 6.1 39.1 18.8 9.1 15 24 15
sum 29.3 9.1 5 20.6 14 6.6 35.8 16.7 8.3 15 24 15

Additional Resources and Comments