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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PLEKHA2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLEKHA2 TAPP2
Sequence Length (AA) Molecular Weight (Da)
425 47255
Protein Name
Pleckstrin homology domain-containing family A member 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPYVDRQNRI CGFLDIEEHE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA
60 70 80 90 100
MGAGAVGALQ LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD
110 120 130 140 150
MKDWVEALNQ ASKITVPKGG GLPMTTEVLK SLAAPPALEK KPQVAYKTEI
160 170 180 190 200
IGGVVVHTPI SQNGGDGQEG SEPGSHTILR RSQSYIPTSG CRASTGPPLI
210 220 230 240 250
KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR TIFLKDVLKT
260 270 280 290 300
HECLVKSGDL LMRDNLFEII TSSRTFYVQA DSPEDMHSWI KEIGAAVQAL
310 320 330 340 350
KCHPRETSFS RSISLTRPGS SSLSSGPNSI LCRGRPPLEE KKALCKAPSV
360 370 380 390 400
ASSWQPWTPV PQAGEKLLPP GDTSEDSLFT PRPGEGAPPG VLPSSRIRHR
410 420 425
SEPQHPKEKP FMFNLDDENI RTSDV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-992 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLEKHA2
Peptide Modified Sequence
SIS[+80.0]LTRPGSSSLSSGPNSILC[+57.0]R
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
314, 332
Peptide - Site of Modification
3, 21
Peptide Start
312
Peptide End
333
CPTAC ID
CPTAC-992
Peptide Molecular Mass
2,355.1254
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16 (2+) 63.8 11.7 10.5 48.7 16.8 12.8 80.3 20.5 16.6 15 24 15
y7 (1+) 55.2 14.8 9.4 48.7 18 12.4 73.6 23.3 15.6 15 24 15
y8 (1+) 37.4 11.6 9 40.5 17.8 10.3 55.1 21.2 13.7 15 24 15
sum 47.3 12 8.8 40.9 17 11.2 62.5 20.8 14.2 15 24 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments