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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PPP1R10 CAT53, FB19, PNUTS
Sequence Length (AA) Molecular Weight (Da)
940 99058
Protein Name
Serine/threonine-protein phosphatase 1 regulatory subunit 10
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSGPIDPKE LLKGLDSFLN RDGEVKSVDG ISKIFSLMKE ARKMVSRCTY
60 70 80 90 100
LNILLQTRSP EILVKFIDVG GYKLLNNWLT YSKTTNNIPL LQQILLTLQH
110 120 130 140 150
LPLTVDHLKQ NNTAKLVKQL SKSSEDEELR KLASVLVSDW MAVIRSQSST
160 170 180 190 200
QPAEKDKKKR KDEGKSRTTL PERPLTEVKA ETRAEEAPEK KREKPKSLRT
210 220 230 240 250
TAPSHAKFRS TGLELETPSL VPVKKNASTV VVSDKYNLKP IPLKRQSNVA
260 270 280 290 300
APGDATPPAE KKYKPLNTTP NATKEIKVKI IPPQPMEGLG FLDALNSAPV
310 320 330 340 350
PGIKIKKKKK VLSPTAAKPS PFEGKTSTEP STAKPSSPEP APPSEAMDAD
360 370 380 390 400
RPGTPVPPVE VPELMDTASL EPGALDAKPV ESPGDPNQLT RKGRKRKSVT
410 420 430 440 450
WPEEGKLREY FYFELDETER VNVNKIKDFG EAAKREILSD RHAFETARRL
460 470 480 490 500
SHDNMEEKVP WVCPRPLVLP SPLVTPGSNS QERYIQAERE KGILQELFLN
510 520 530 540 550
KESPHEPDPE PYEPIPPKLI PLDEECSMDE TPYVETLEPG GSGGSPDGAG
560 570 580 590 600
GSKLPPVLAN LMGSMGAGKG PQGPGGGGIN VQEILTSIMG SPNSHPSEEL
610 620 630 640 650
LKQPDYSDKI KQMLVPHGLL GPGPIANGFP PGGPGGPKGM QHFPPGPGGP
660 670 680 690 700
MPGPHGGPGG PVGPRLLGPP PPPRGGDPFW DGPGDPMRGG PMRGGPGPGP
710 720 730 740 750
GPYHRGRGGR GGNEPPPPPP PFRGARGGRS GGGPPNGRGG PGGGMVGGGG
760 770 780 790 800
HRPHEGPGGG MGNSSGHRPH EGPGGGMGSG HRPHEGPGGS MGGGGGHRPH
810 820 830 840 850
EGPGGGISGG SGHRPHEGPG GGMGAGGGHR PHEGPGGSMG GSGGHRPHEG
860 870 880 890 900
PGHGGPHGHR PHDVPGHRGH DHRGPPPHEH RGHDGPGHGG GGHRGHDGGH
910 920 930 940
SHGGDMSNRP VCRHFMMKGN CRYENNCAFY HPGVNGPPLP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-993 Collapse assay details

Data source: Panorama

Official Gene Symbol
PPP1R10
Peptide Modified Sequence
VLS[+80.0]PTAAKPSPFEGK
Modification Type
Phospho (ST)
Protein - Site of Modification
313
Peptide - Site of Modification
3
Peptide Start
311
Peptide End
325
CPTAC ID
CPTAC-993
Peptide Molecular Mass
1,607.7960
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 23.7 6.5 8.3 19.8 7.8 9.4 30.9 10.2 12.5 15 24 15
y13-98 (2+) 27.6 7.3 8 15.7 7.9 8.6 31.8 10.8 11.7 15 24 15
y13 (2+) 30.4 9 9.1 31 16.3 14.4 43.4 18.6 17 15 24 15
sum 26.6 7 8.2 21.7 9.1 10.6 34.3 11.5 13.4 15 24 15

Additional Resources and Comments