PPP1R10, CPTAC-993 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PPP1R10	CAT53, FB19, PNUTS

Sequence Length (AA)	Molecular Weight (Da)
940	99058

Protein Name
Serine/threonine-protein phosphatase 1 regulatory subunit 10

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGSGPIDPKE	LLKGLDSFLN	RDGEVKSVDG	ISKIFSLMKE	ARKMVSRCTY
60	70	80	90	100
LNILLQTRSP	EILVKFIDVG	GYKLLNNWLT	YSKTTNNIPL	LQQILLTLQH
110	120	130	140	150
LPLTVDHLKQ	NNTAKLVKQL	SKSSEDEELR	KLASVLVSDW	MAVIRSQSST
160	170	180	190	200
QPAEKDKKKR	KDEGKSRTTL	PERPLTEVKA	ETRAEEAPEK	KREKPKSLRT
210	220	230	240	250
TAPSHAKFRS	TGLELETPSL	VPVKKNASTV	VVSDKYNLKP	IPLKRQSNVA
260	270	280	290	300
APGDATPPAE	KKYKPLNTTP	NATKEIKVKI	IPPQPMEGLG	FLDALNSAPV
310	320	330	340	350
PGIKIKKKKK	VLSPTAAKPS	PFEGKTSTEP	STAKPSSPEP	APPSEAMDAD
360	370	380	390	400
RPGTPVPPVE	VPELMDTASL	EPGALDAKPV	ESPGDPNQLT	RKGRKRKSVT
410	420	430	440	450
WPEEGKLREY	FYFELDETER	VNVNKIKDFG	EAAKREILSD	RHAFETARRL
460	470	480	490	500
SHDNMEEKVP	WVCPRPLVLP	SPLVTPGSNS	QERYIQAERE	KGILQELFLN
510	520	530	540	550
KESPHEPDPE	PYEPIPPKLI	PLDEECSMDE	TPYVETLEPG	GSGGSPDGAG
560	570	580	590	600
GSKLPPVLAN	LMGSMGAGKG	PQGPGGGGIN	VQEILTSIMG	SPNSHPSEEL
610	620	630	640	650
LKQPDYSDKI	KQMLVPHGLL	GPGPIANGFP	PGGPGGPKGM	QHFPPGPGGP
660	670	680	690	700
MPGPHGGPGG	PVGPRLLGPP	PPPRGGDPFW	DGPGDPMRGG	PMRGGPGPGP
710	720	730	740	750
GPYHRGRGGR	GGNEPPPPPP	PFRGARGGRS	GGGPPNGRGG	PGGGMVGGGG
760	770	780	790	800
HRPHEGPGGG	MGNSSGHRPH	EGPGGGMGSG	HRPHEGPGGS	MGGGGGHRPH
810	820	830	840	850
EGPGGGISGG	SGHRPHEGPG	GGMGAGGGHR	PHEGPGGSMG	GSGGHRPHEG
860	870	880	890	900
PGHGGPHGHR	PHDVPGHRGH	DHRGPPPHEH	RGHDGPGHGG	GGHRGHDGGH
910	920	930	940
SHGGDMSNRP	VCRHFMMKGN	CRYENNCAFY	HPGVNGPPLP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-993 Collapse assay details

Data source: Panorama

Official Gene Symbol: PPP1R10
Peptide Modified Sequence: VLS[+80.0]PTAAKPSPFEGK
Modification Type: Phospho (ST)
Protein - Site of Modification: 313
Peptide - Site of Modification: 3
Peptide Start: 311
Peptide End: 325

CPTAC ID: CPTAC-993
Peptide Molecular Mass: 1,607.7960
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	23.7	6.5	8.3	19.8	7.8	9.4	30.9	10.2	12.5	15	24	15
y13-98 (2+)	27.6	7.3	8	15.7	7.9	8.6	31.8	10.8	11.7	15	24	15
y13 (2+)	30.4	9	9.1	31	16.3	14.4	43.4	18.6	17	15	24	15
sum	26.6	7	8.2	21.7	9.1	10.6	34.3	11.5	13.4	15	24	15

PPP1R10

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-993 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PPP1R10

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-993 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments