Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINA3 AACT
Sequence Length (AA) Molecular Weight (Da)
423 47651
Protein Name
Alpha-1-antichymotrypsin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MERMLPLLAL GLLAAGFCPA VLCHPNSPLD EENLTQENQD RGTHVDLGLA
60 70 80 90 100
SANVDFAFSL YKQLVLKAPD KNVIFSPLSI STALAFLSLG AHNTTLTEIL
110 120 130 140 150
KGLKFNLTET SEAEIHQSFQ HLLRTLNQSS DELQLSMGNA MFVKEQLSLL
160 170 180 190 200
DRFTEDAKRL YGSEAFATDF QDSAAAKKLI NDYVKNGTRG KITDLIKDLD
210 220 230 240 250
SQTMMVLVNY IFFKAKWEMP FDPQDTHQSR FYLSKKKWVM VPMMSLHHLT
260 270 280 290 300
IPYFRDEELS CTVVELKYTG NASALFILPD QDKMEEVEAM LLPETLKRWR
310 320 330 340 350
DSLEFREIGE LYLPKFSISR DYNLNDILLQ LGIEEAFTSK ADLSGITGAR
360 370 380 390 400
NLAVSQVVHK AVLDVFEEGT EASAATAVKI TLLSALVETR TIVRFNRPFL
410 420 423
MIIVPTDTQN IFFMSKVTNP KQA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-1062 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA3
Peptide Sequence
EIGELYLPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
307
Peptide End
315
CPTAC ID
non-CPTAC-1062
Peptide Molecular Mass
1,060.5805
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 3.6 4 4.7 6.1 5.8 5.4 7.1 7 7.2 15 15 15
y2 (1+) 4 3.1 3.6 8.5 6 6.1 9.4 6.8 7.1 15 15 15
y3 (1+) 6.4 3 4.2 9.5 4 4.4 11.5 5 6.1 15 15 15
y5 (1+) 4.4 4.7 3.5 8 6 3.9 9.1 7.6 5.2 15 15 15
y7 (1+) 5.8 3.2 5.3 10.6 5.4 5.7 12.1 6.3 7.8 15 15 15
sum 2.9 1.4 2.7 7.3 4.5 3 7.9 4.7 4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1061 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA3
Peptide Sequence
NLAVSQVVHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
351
Peptide End
360
CPTAC ID
non-CPTAC-1061
Peptide Molecular Mass
1,093.6244
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
b2 (1+) 3 8.4 6.4 9.2 8.6 6.8 9.7 12 9.3 4 15 15
y6 (2+) 12.9 8.3 6.5 15 8.6 7.9 19.8 12 10.2 15 15 15
y7 (2+) 13.9 13.3 10.6 16.3 13.7 9.8 21.4 19.1 14.4 15 15 15
y8 (2+) 8.2 11.5 8 12.5 12.1 7.2 14.9 16.7 10.8 15 15 15
sum 6.6 8.4 6 11.3 9.4 5.6 13.1 12.6 8.2 15 15 15

Additional Resources and Comments