SERPINA3, CPTAC-1062 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SERPINA3	AACT

Sequence Length (AA)	Molecular Weight (Da)
423	47651

Protein Name
Alpha-1-antichymotrypsin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MERMLPLLAL	GLLAAGFCPA	VLCHPNSPLD	EENLTQENQD	RGTHVDLGLA
60	70	80	90	100
SANVDFAFSL	YKQLVLKAPD	KNVIFSPLSI	STALAFLSLG	AHNTTLTEIL
110	120	130	140	150
KGLKFNLTET	SEAEIHQSFQ	HLLRTLNQSS	DELQLSMGNA	MFVKEQLSLL
160	170	180	190	200
DRFTEDAKRL	YGSEAFATDF	QDSAAAKKLI	NDYVKNGTRG	KITDLIKDLD
210	220	230	240	250
SQTMMVLVNY	IFFKAKWEMP	FDPQDTHQSR	FYLSKKKWVM	VPMMSLHHLT
260	270	280	290	300
IPYFRDEELS	CTVVELKYTG	NASALFILPD	QDKMEEVEAM	LLPETLKRWR
310	320	330	340	350
DSLEFREIGE	LYLPKFSISR	DYNLNDILLQ	LGIEEAFTSK	ADLSGITGAR
360	370	380	390	400
NLAVSQVVHK	AVLDVFEEGT	EASAATAVKI	TLLSALVETR	TIVRFNRPFL
410	420	423
MIIVPTDTQN	IFFMSKVTNP	KQA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1062 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA3
Peptide Sequence: EIGELYLPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 307
Peptide End: 315

CPTAC ID: non-CPTAC-1062
Peptide Molecular Mass: 1,060.5805
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (2+)	3.6	4	4.7	6.1	5.8	5.4	7.1	7	7.2	15	15	15
y2 (1+)	4	3.1	3.6	8.5	6	6.1	9.4	6.8	7.1	15	15	15
y3 (1+)	6.4	3	4.2	9.5	4	4.4	11.5	5	6.1	15	15	15
y5 (1+)	4.4	4.7	3.5	8	6	3.9	9.1	7.6	5.2	15	15	15
y7 (1+)	5.8	3.2	5.3	10.6	5.4	5.7	12.1	6.3	7.8	15	15	15
sum	2.9	1.4	2.7	7.3	4.5	3	7.9	4.7	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1061 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA3
Peptide Sequence: NLAVSQVVHK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 351
Peptide End: 360

CPTAC ID: non-CPTAC-1061
Peptide Molecular Mass: 1,093.6244
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers