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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

A2M

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
A2M CPAMD5
Sequence Length (AA) Molecular Weight (Da)
1474 163291
Protein Name
Alpha-2-macroglobulin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGKNKLLHPS LVLLLLVLLP TDASVSGKPQ YMVLVPSLLH TETTEKGCVL
60 70 80 90 100
LSYLNETVTV SASLESVRGN RSLFTDLEAE NDVLHCVAFA VPKSSSNEEV
110 120 130 140 150
MFLTVQVKGP TQEFKKRTTV MVKNEDSLVF VQTDKSIYKP GQTVKFRVVS
160 170 180 190 200
MDENFHPLNE LIPLVYIQDP KGNRIAQWQS FQLEGGLKQF SFPLSSEPFQ
210 220 230 240 250
GSYKVVVQKK SGGRTEHPFT VEEFVLPKFE VQVTVPKIIT ILEEEMNVSV
260 270 280 290 300
CGLYTYGKPV PGHVTVSICR KYSDASDCHG EDSQAFCEKF SGQLNSHGCF
310 320 330 340 350
YQQVKTKVFQ LKRKEYEMKL HTEAQIQEEG TVVELTGRQS SEITRTITKL
360 370 380 390 400
SFVKVDSHFR QGIPFFGQVR LVDGKGVPIP NKVIFIRGNE ANYYSNATTD
410 420 430 440 450
EHGLVQFSIN TTNVMGTSLT VRVNYKDRSP CYGYQWVSEE HEEAHHTAYL
460 470 480 490 500
VFSPSKSFVH LEPMSHELPC GHTQTVQAHY ILNGGTLLGL KKLSFYYLIM
510 520 530 540 550
AKGGIVRTGT HGLLVKQEDM KGHFSISIPV KSDIAPVARL LIYAVLPTGD
560 570 580 590 600
VIGDSAKYDV ENCLANKVDL SFSPSQSLPA SHAHLRVTAA PQSVCALRAV
610 620 630 640 650
DQSVLLMKPD AELSASSVYN LLPEKDLTGF PGPLNDQDNE DCINRHNVYI
660 670 680 690 700
NGITYTPVSS TNEKDMYSFL EDMGLKAFTN SKIRKPKMCP QLQQYEMHGP
710 720 730 740 750
EGLRVGFYES DVMGRGHARL VHVEEPHTET VRKYFPETWI WDLVVVNSAG
760 770 780 790 800
VAEVGVTVPD TITEWKAGAF CLSEDAGLGI SSTASLRAFQ PFFVELTMPY
810 820 830 840 850
SVIRGEAFTL KATVLNYLPK CIRVSVQLEA SPAFLAVPVE KEQAPHCICA
860 870 880 890 900
NGRQTVSWAV TPKSLGNVNF TVSAEALESQ ELCGTEVPSV PEHGRKDTVI
910 920 930 940 950
KPLLVEPEGL EKETTFNSLL CPSGGEVSEE LSLKLPPNVV EESARASVSV
960 970 980 990 1000
LGDILGSAMQ NTQNLLQMPY GCGEQNMVLF APNIYVLDYL NETQQLTPEI
1010 1020 1030 1040 1050
KSKAIGYLNT GYQRQLNYKH YDGSYSTFGE RYGRNQGNTW LTAFVLKTFA
1060 1070 1080 1090 1100
QARAYIFIDE AHITQALIWL SQRQKDNGCF RSSGSLLNNA IKGGVEDEVT
1110 1120 1130 1140 1150
LSAYITIALL EIPLTVTHPV VRNALFCLES AWKTAQEGDH GSHVYTKALL
1160 1170 1180 1190 1200
AYAFALAGNQ DKRKEVLKSL NEEAVKKDNS VHWERPQKPK APVGHFYEPQ
1210 1220 1230 1240 1250
APSAEVEMTS YVLLAYLTAQ PAPTSEDLTS ATNIVKWITK QQNAQGGFSS
1260 1270 1280 1290 1300
TQDTVVALHA LSKYGAATFT RTGKAAQVTI QSSGTFSSKF QVDNNNRLLL
1310 1320 1330 1340 1350
QQVSLPELPG EYSMKVTGEG CVYLQTSLKY NILPEKEEFP FALGVQTLPQ
1360 1370 1380 1390 1400
TCDEPKAHTS FQISLSVSYT GSRSASNMAI VDVKMVSGFI PLKPTVKMLE
1410 1420 1430 1440 1450
RSNHVSRTEV SSNHVLIYLD KVSNQTLSLF FTVLQDVPVR DLKPAIVKVY
1460 1470 1474
DYYETDEFAI AEYNAPCSKD LGNA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1068 Collapse assay details

Data source: Panorama

Official Gene Symbol
A2M
Peptide Sequence
AIGYLNTGYQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1004
Peptide End
1014
CPTAC ID
non-CPTAC-1068
Peptide Molecular Mass
1,254.6357
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 8.5 4.9 5.2 10.2 7 5.5 13.3 8.5 7.6 15 15 15
y9 (2+) 11.5 4.9 4.7 8.8 5.9 5.4 14.5 7.7 7.2 15 15 15
y4 (1+) 6.9 2.9 4.1 13.3 6.9 3.7 15 7.5 5.5 15 15 15
y6 (1+) 6.5 4.5 4.6 9.5 6.2 5.3 11.5 7.7 7 15 15 15
y7 (1+) 8 3.8 6.1 7.1 3.3 7.8 10.7 5 9.9 15 15 15
sum 4.2 2.7 2.8 6.6 4.1 3.8 7.8 4.9 4.7 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-3384 Collapse assay details

Data source: Panorama

Official Gene Symbol
A2m
Peptide Sequence
IQEEGTGVEETGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
332
Peptide End
344
CPTAC ID
non-CPTAC-3384
Peptide Molecular Mass
1,375.6467
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.2 6.5 7.2 16.6 7.6 6.9 17.7 10 10 15 15 15
y2 (1+) 9 6.2 6.8 20.3 10.3 8 22.2 12 10.5 15 15 15
y3 (1+) 8.6 7.2 9.7 16.6 10.5 8.5 18.7 12.7 12.9 15 15 15
y9 (1+) 12.7 9 6.5 18 11.4 7.5 22 14.5 9.9 15 15 15
y11 (1+) 7.5 7.9 9.7 17 9.1 9.7 18.6 12.1 13.7 15 15 15
sum 3.3 4.3 4.2 15.8 7 4.7 16.1 8.2 6.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3311 Collapse assay details

Data source: Panorama

Official Gene Symbol
A2m
Peptide Sequence
VTLPTVPGDYTAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1302
Peptide End
1315
CPTAC ID
non-CPTAC-3311
Peptide Molecular Mass
1,360.7238
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 19 9 6.7 23.1 12.2 5.7 29.9 15.2 8.8 15 15 15
y7 (1+) 19.7 6.4 7.4 19.2 8.8 6.2 27.5 10.9 9.7 15 15 15
y10 (2+) 12.2 7.1 4.6 12.3 7.4 6.5 17.3 10.3 8 15 15 15
b2 (1+) 28.1 17.9 16.4 25.7 18.9 14.6 38.1 26 22 15 15 15
y10 (1+) 13.9 5.3 5.3 15.4 4.6 7 20.7 7 8.8 15 15 15
sum 8.2 3.5 2.3 11 4.9 3.9 13.7 6 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments