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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINC1 AT3
Sequence Length (AA) Molecular Weight (Da)
464 52602
Protein Name
Antithrombin-III
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYSNVIGTVT SGKRKVYLLS LLLIGFWDCV TCHGSPVDIC TAKPRDIPMN
60 70 80 90 100
PMCIYRSPEK KATEDEGSEQ KIPEATNRRV WELSKANSRF ATTFYQHLAD
110 120 130 140 150
SKNDNDNIFL SPLSISTAFA MTKLGACNDT LQQLMEVFKF DTISEKTSDQ
160 170 180 190 200
IHFFFAKLNC RLYRKANKSS KLVSANRLFG DKSLTFNETY QDISELVYGA
210 220 230 240 250
KLQPLDFKEN AEQSRAAINK WVSNKTEGRI TDVIPSEAIN ELTVLVLVNT
260 270 280 290 300
IYFKGLWKSK FSPENTRKEL FYKADGESCS ASMMYQEGKF RYRRVAEGTQ
310 320 330 340 350
VLELPFKGDD ITMVLILPKP EKSLAKVEKE LTPEVLQEWL DELEEMMLVV
360 370 380 390 400
HMPRFRIEDG FSLKEQLQDM GLVDLFSPEK SKLPGIVAEG RDDLYVSDAF
410 420 430 440 450
HKAFLEVNEE GSEAAASTAV VIAGRSLNPN RVTFKANRPF LVFIREVPLN
460 464
TIIFMGRVAN PCVK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1070 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINC1
Peptide Sequence
DDLYVSDAFHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
393
Peptide End
403
CPTAC ID
non-CPTAC-1070
Peptide Molecular Mass
1,308.5986
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 5.3 2.6 2.1 8.9 4 2.5 10.4 4.8 3.3 15 15 15
b2 (1+) 4.3 1.8 2.9 6.9 4.9 3.1 8.1 5.2 4.2 15 15 15
y3 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
y8 (2+) 4 2.6 3.4 8.1 5.7 3.6 9 6.3 5 15 15 15
y9 (2+) 4.5 4 3 6.6 5.5 3.7 8 6.8 4.8 15 15 15
sum 2.2 2.5 2.6 6.5 5 3 6.9 5.6 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3338 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinc1
Peptide Sequence
DDLYVSDAFHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
393
Peptide End
403
CPTAC ID
non-CPTAC-3338
Peptide Molecular Mass
1,308.5986
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 7.1 8.4 7.1 10.5 9.7 11.2 12.7 12.8 13.3 15 15 15
b2 (1+) 6.6 8.1 5.9 12.6 7.9 8.3 14.2 11.3 10.2 15 15 15
y3 (2+) 129.4 45.8 60 134.3 68.1 97.8 186.5 82.1 114.7 15 15 15
y8 (2+) 5.7 3 5 13.7 5.4 10 14.8 6.2 11.2 15 15 15
y9 (2+) 6.2 7.2 6.9 13.9 6.6 9.3 15.2 9.8 11.6 15 15 15
sum 3.8 3.1 3.5 12.3 3.8 7.6 12.9 4.9 8.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1071 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINC1
Peptide Sequence
FATTFYQHLADSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
90
Peptide End
102
CPTAC ID
non-CPTAC-1071
Peptide Molecular Mass
1,527.7358
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.9 6.3 3.6 7.4 7.4 4 9.5 9.7 5.4 15 15 15
y9 (2+) 10.8 4.7 6 9.4 6 6.3 14.3 7.6 8.7 15 15 15
y10 (2+) 8.7 7.2 5.3 17.3 8.7 6.6 19.4 11.3 8.5 15 15 15
y11 (2+) 7.4 7.5 5.8 8.3 10.1 6.5 11.1 12.6 8.7 15 15 15
y2 (1+) 5.1 4.2 4.4 10 9.3 5 11.2 10.2 6.7 15 15 15
sum 3.5 4.2 3.1 7 7 2.1 7.8 8.2 3.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1072 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINC1
Peptide Sequence
TSDQIHFFFAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
147
Peptide End
157
CPTAC ID
non-CPTAC-1072
Peptide Molecular Mass
1,339.6561
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 6.7 4.8 4.3 10.7 5.9 4.6 12.6 7.6 6.3 15 15 15
y10 (2+) 4.8 4.7 5.4 11.6 7.3 5.6 12.6 8.7 7.8 15 15 15
y2 (1+) 7 6.5 3.8 11.8 7 4.2 13.7 9.6 5.7 15 15 15
y5 (1+) 7.7 6.9 4 10.9 6.3 4.3 13.3 9.3 5.9 15 15 15
y6 (1+) 6.4 4.1 5.5 12.6 5.4 5.1 14.1 6.8 7.5 15 15 15
sum 3.9 2.6 3.4 10.1 4.8 3.1 10.8 5.5 4.6 15 15 15


Additional Resources and Comments