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Overview Data source: UniProt

Official Gene Symbol Other Aliases
APOA4 N/A
Sequence Length (AA) Molecular Weight (Da)
396 45399
Protein Name
Apolipoprotein A-IV
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFLKAVVLTL ALVAVAGARA EVSADQVATV MWDYFSQLSN NAKEAVEHLQ
60 70 80 90 100
KSELTQQLNA LFQDKLGEVN TYAGDLQKKL VPFATELHER LAKDSEKLKE
110 120 130 140 150
EIGKELEELR ARLLPHANEV SQKIGDNLRE LQQRLEPYAD QLRTQVNTQA
160 170 180 190 200
EQLRRQLTPY AQRMERVLRE NADSLQASLR PHADELKAKI DQNVEELKGR
210 220 230 240 250
LTPYADEFKV KIDQTVEELR RSLAPYAQDT QEKLNHQLEG LTFQMKKNAE
260 270 280 290 300
ELKARISASA EELRQRLAPL AEDVRGNLRG NTEGLQKSLA ELGGHLDQQV
310 320 330 340 350
EEFRRRVEPY GENFNKALVQ QMEQLRQKLG PHAGDVEGHL SFLEKDLRDK
360 370 380 390 396
VNSFFSTFKE KESQDKTLSL PELEQQQEQQ QEQQQEQVQM LAPLES

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1079 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOA4
Peptide Sequence
LGEVNTYAGDLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
66
Peptide End
78
CPTAC ID
non-CPTAC-1079
Peptide Molecular Mass
1,406.7042
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 9.8 4.7 5.2 13.5 6.7 5.3 16.7 8.2 7.4 15 15 15
y6 (1+) 12.7 6.2 4.2 13.7 7.3 5.6 18.7 9.6 7 15 15 15
y7 (1+) 10.4 5.8 3.3 10.6 7.4 4.6 14.8 9.4 5.7 15 15 15
y8 (1+) 15.4 3.8 4.7 18.6 6.9 6.9 24.1 7.9 8.3 15 15 15
y9 (1+) 10.8 8.6 5.5 15.6 8.6 5.1 19 12.2 7.5 15 15 15
sum 5.4 3.4 2.6 10 4.4 3.2 11.4 5.6 4.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1077 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOA4
Peptide Sequence
LLPHANEVSQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
113
Peptide End
123
CPTAC ID
non-CPTAC-1077
Peptide Molecular Mass
1,234.6670
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.9 3.6 3.3 8.9 8.7 5 10.2 9.4 6 15 15 15
y4 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
y8 (2+) 8.8 4.9 4.5 12 7.7 4.5 14.9 9.1 6.4 15 15 15
y9 (2+) 5.4 4 4.2 8.7 5 5.6 10.2 6.4 7 15 15 15
b4 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
sum 4.9 3 3.2 8.3 5.5 4.3 9.6 6.3 5.4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1078 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOA4
Peptide Sequence
SLAPYAQDTQEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
222
Peptide End
233
CPTAC ID
non-CPTAC-1078
Peptide Molecular Mass
1,349.6463
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 16.8 8.3 6.3 16.9 9.7 7.2 23.8 12.8 9.6 15 15 15
b2 (1+) 12.1 7.1 5.8 19.8 11.9 6.7 23.2 13.9 8.9 15 15 15
y9 (2+) 10.8 9.1 3.9 11.3 10.8 5.5 15.6 14.1 6.7 15 15 15
y10 (2+) 17.8 8.5 6.7 21 7.9 6.8 27.5 11.6 9.5 15 15 15
y9 (1+) 28 9.1 9.2 33.5 11.6 9.9 43.7 14.7 13.5 15 15 15
sum 7.4 5.1 2.6 9.1 7.8 3.4 11.7 9.3 4.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3411 Collapse assay details

Data source: Panorama

Official Gene Symbol
Apoa4
Peptide Sequence
SLEDLNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
288
Peptide End
294
CPTAC ID
non-CPTAC-3411
Peptide Molecular Mass
845.4243
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 8.2 9.1 8.7 13 11.3 7.6 15.4 14.5 11.6 15 15 15
b2 (1+) 8 7.8 11.3 16.4 7.6 10.1 18.2 10.9 15.2 15 15 15
y3 (1+) 7.1 10.2 7.2 22.1 11.8 8.7 23.2 15.6 11.3 15 15 15
y4 (1+) 6.9 7.4 7 21 11.1 9.8 22.1 13.3 12 15 15 15
y5 (1+) 9.7 10.8 6.6 14.2 10.9 10.5 17.2 15.3 12.4 15 15 15
sum 5.3 5.6 3.6 14.7 8 7.4 15.6 9.8 8.2 15 15 15

Additional Resources and Comments