APOC1, CPTAC-1085 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
APOC1	N/A

Sequence Length (AA)	Molecular Weight (Da)
83	9332

Protein Name
Apolipoprotein C-I

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRLFLSLPVL	VVVLSIVLEG	PAPAQGTPDV	SSALDKLKEF	GNTLEDKARE
60	70	80	83
LISRIKQSEL	SAKMREWFSE	TFQKVKEKLK	IDS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2623 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOC1
Peptide Sequence: EWFSETFQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 74

CPTAC ID: non-CPTAC-2623
Peptide Molecular Mass: 1,200.5451
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	12.2	5.1	7.9	15.8	6.4	8.3	20	8.2	11.5	15	15	15
y6 (1+)	12.1	6.8	6.6	16.6	8	6.8	20.5	10.5	9.5	15	15	15
y7 (2+)	25.2	6.1	8.5	30.2	8.5	8.2	39.3	10.5	11.8	15	15	15
y4 (1+)	20.9	8.6	4.2	31.1	10.9	4.3	37.5	13.9	6	15	15	15
y5 (1+)	18.1	10	6	17.8	9.9	8	25.4	14.1	10	15	15	15
sum	6.7	3.4	4.6	14.7	6.1	4.6	16.2	7	6.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1085 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOC1
Peptide Sequence: TPDVSSALDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 27
Peptide End: 36

CPTAC ID: non-CPTAC-1085
Peptide Molecular Mass: 1,031.5135
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers