APOC2, CPTAC-1084 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
APOC2	APC2

Sequence Length (AA)	Molecular Weight (Da)
101	11284

Protein Name
Apolipoprotein C-II

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGTRLLPALF	LVLLVLGFEV	QGTQQPQQDE	MPSPTFLTQV	KESLSSYWES
60	70	80	90	100
AKTAAQNLYE	KTYLPAVDEK	LRDLYSKSTA	AMSTYTGIFT	DQVLSVLKGE
101
E

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-2625 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOC2
Peptide Sequence: ESLSSYWESAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 42
Peptide End: 52

CPTAC ID: non-CPTAC-2625
Peptide Molecular Mass: 1,285.5826
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	8.6	3.8	8.6	13.6	6.8	7.6	16.1	7.8	11.5	15	15	15
y3 (1+)	12.9	4	5.5	14.5	5	5.5	19.4	6.4	7.8	15	15	15
b2 (1+)	17.4	11.7	8.8	18.6	12.1	8.5	25.5	16.8	12.2	15	15	15
y7 (1+)	12.1	6.5	6.8	18.7	7.3	7.2	22.3	9.8	9.9	15	15	15
sum	6.3	3.7	5.2	11.7	5.7	4.5	13.3	6.8	6.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1084 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOC2
Peptide Sequence: TYLPAVDEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 62
Peptide End: 70

CPTAC ID: non-CPTAC-1084
Peptide Molecular Mass: 1,034.5284
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers