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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
APOC3 N/A
Sequence Length (AA) Molecular Weight (Da)
99 10852
Protein Name
Apolipoprotein C-III
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPRVLLVVA LLALLASARA SEAEDASLLS FMQGYMKHAT KTAKDALSSV
60 70 80 90 99
QESQVAQQAR GWVTDGFSSL KDYWSTVKDK FSEFWDLDPE VRPTSAVAA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1083 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOC3
Peptide Sequence
GWVTDGFSSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
61
Peptide End
71
CPTAC ID
non-CPTAC-1083
Peptide Molecular Mass
1,195.5873
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.3 3.5 5.9 10.3 4.6 6.5 12.1 5.8 8.8 15 15 15
b2 (1+) 4.5 3.6 2.4 10.3 5.6 3.2 11.2 6.7 4 15 15 15
y7 (1+) 5.3 6 4.4 8.3 7.8 5.7 9.8 9.8 7.2 15 15 15
y8 (1+) 5.2 2.9 5.4 16.4 4.6 5.6 17.2 5.4 7.8 15 15 15
y9 (1+) 8.2 2.9 6.7 12.6 5.4 5.8 15 6.1 8.9 15 15 15
sum 3.3 2.4 2.6 11.1 4.3 2.5 11.6 4.9 3.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-4017 Collapse assay details

Data source: Panorama

Official Gene Symbol
Apoc3
Peptide Sequence
TVQDALSSVQESDIAVVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
42
Peptide End
60
CPTAC ID
non-CPTAC-4017
Peptide Molecular Mass
1,987.0222
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.8 10.1 1.9 11.6 10.7 5.8 14.6 14.7 6.1 15 15 15
y5 (1+) 5.2 3 2.9 7.7 6.2 5.1 9.3 6.9 5.9 15 15 15
y4 (1+) 5.2 5.5 4 8.3 9.3 4.9 9.8 10.8 6.3 15 15 15
y3 (1+) 5.9 3.9 1.7 7.7 7.3 4.6 9.7 8.3 4.9 15 15 15
b15 (2+) 6.4 3 4.8 6.9 6.8 6.3 9.4 7.4 7.9 15 15 15
sum 3.1 2.4 1.4 6 5.8 4 6.8 6.3 4.2 15 15 15


Additional Resources and Comments