Apoc3, CPTAC-4017 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Apoc3	N/A

Sequence Length (AA)	Molecular Weight (Da)
99	10982

Protein Name
Apolipoprotein C-III

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQPRTLLTVA	LLALLASARA	EEVEGSLLLG	SVQGYMEQAS	KTVQDALSSV
60	70	80	90	99
QESDIAVVAR	GWMDNHFRFL	KGYWSKFTDK	FTGFWDSNPE	DQPTPAIES

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1083 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOC3
Peptide Sequence: GWVTDGFSSLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 61
Peptide End: 71

CPTAC ID: non-CPTAC-1083
Peptide Molecular Mass: 1,195.5873
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	6.3	3.5	5.9	10.3	4.6	6.5	12.1	5.8	8.8	15	15	15
b2 (1+)	4.5	3.6	2.4	10.3	5.6	3.2	11.2	6.7	4	15	15	15
y7 (1+)	5.3	6	4.4	8.3	7.8	5.7	9.8	9.8	7.2	15	15	15
y8 (1+)	5.2	2.9	5.4	16.4	4.6	5.6	17.2	5.4	7.8	15	15	15
y9 (1+)	8.2	2.9	6.7	12.6	5.4	5.8	15	6.1	8.9	15	15	15
sum	3.3	2.4	2.6	11.1	4.3	2.5	11.6	4.9	3.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-4017 Collapse assay details

Data source: Panorama

Official Gene Symbol: Apoc3
Peptide Sequence: TVQDALSSVQESDIAVVAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 42
Peptide End: 60

CPTAC ID: non-CPTAC-4017
Peptide Molecular Mass: 1,987.0222
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers